DA到Lewis大鼠肝脏移植急性排斥模型的建立
肝移植；移植；排斥反应；同种；大鼠；近交系,,肝移植；移植；排斥反应；同种；大鼠；近交系,0引言,1材料和方法,2结果,3讨论,【参考文献】
【Abstract】 AIM: To establish an acute rejection model of DAtoLewis rat liver transplantation (LT) and observe the characteristics of acute rejection. METHODS: Thirty LT models were established by modified Kamada twocuff technique, with inbred male DA rats as the donors and inbred male Lewis rats as the recipients. The recipients were randomly divided into 2 groups: control group (n=15) without immunosuppressive agent treatment after LT and FK506 treatment group (n=15) lavaged with 0.2 mg/(kg·d) FK506 from 1 to 7 d after LT. Every 3 recipients were sacrificed respectively at day 4, 7, 10 after LT to detect the changes of serum biochemistry [aspartate aminotransferase (AST) and total bilirubin (BILI)] and the grafts histology. The other 6 recipients were left in each group to observe the animal survival time. RESULTS: The serum concentrations of AST and BILI in control group were higher than in FK506 treatment group at day 7 and 10 after LT [AST: 7 d, (17.7±3.6) μkat/L vs (7.9±1.6) μkat/L, P<0.05; 10 d, (21.9±4.3) μkat/L vs (4.7±0.7) μkat/L, P<0.05. BILI: 7 d, (90.9±18.8) μmol/L vs (6.8±0.7) μmol/L, P<0.05; 10 d, (111.5±23.3) μmol/L vs (15.5±3.2) μmol/L, P<0.05]. In control group, the histological manifestations of the grafts were obvious rejection at day 7, and became more sever at day 10. In FK506 treatment group, the histological manifestations were mild or uncertain rejection at day 7 and 10. In control group, the median survival time was 12 d and the 95% confidence interval was (11.77-13.90) d (n=6, 12 d×4, 14 d×1, 15 d×1). In FK506 treatment group, the median survival time was 78 d and the 95% confidence interval was (65.26-90.74) d (n=6, >65 d×1, >67 d×1, 76 d, 78 d, 89 d, 90 d). FK506 treatment group has the longer survival time than control group (P<0.05). CONCLUSION: DAtoLewis rat strain combination is a stable rejection model of rat liver transplantation. The features of this model are similar to the clinical rejection that can be reversed by immunosuppressive agent FK506. ......
您现在查看是摘要页，全文长 9546 字符。