【摘要】 目的 比较子芩和枯芩中黄芩苷的含量。方法 采用反相高效液相色谱法测定含量，色谱柱为十八烷基硅烷键合硅胶柱；流动相为乙腈-水-冰醋酸(35∶65∶1)；检测波长为280nm。结果 在0.068～1.36μg范围内呈良好的线性关系；平均回收率为97.60%，RSD＝1.16%。黄芩苷的含量：(1)购自成都五块石中药材市场：子芩15.57%、枯芩4.82%(枯朽部分0.85%、未枯朽部分13.31%)；(2)购自湖南省药材公司：子芩13.06%、枯芩5.19%(枯朽部分2.02%、未枯朽部分12.48%)；(3)购自邵东廉桥中药材市场：子芩14.96%、枯芩7.43%(枯朽部分1.68%、未枯朽部分14.15%)。结论 子芩质量优于枯芩；枯芩中黄芩苷含量低主要是由于黄芩枯朽造成的。

     【关键词】 RP-HPLC；黄芩；黄芩苷；含量测定

    Study on determination of Baicalin between ziqin and kuqin

    ZHOU Xiao-jiang，LI Xue-jing，GUO Chun.Hunan College of TCM,Changsha 410004,China

    【Abstract】 Objective To study the content determination of Baicalin between ziqin and kuqin.Methods RP-HPLC analysis was carried out,Baicalin was separated completely on an C18 column,using a mixture of acetonitrile,water and glacial acetic acid(35∶65∶1) as mobile phase,with UV detection at 280nm.Results Baicalin has better linear correlation between 0.068μg and 1.36μg ......