HAb18G_CD147；癌_肝细胞；βigh3

βigh3在肝癌细胞系中差异表达的筛选和鉴定

http://www.100md.com 《第四军医大学学报》 2006年第12期

HAb18G,CD147；癌,肝细胞；βigh3,TGFBI；肿瘤转移,,HAb18G,CD147；癌，肝细胞；βigh3,TGFBI；肿瘤转移,βigh3在肝癌细胞系中差异表达的筛选和鉴

【Abstract】 AIM: To screen and identify the differential expression of TGFβinduced molecule βigh3 in hepatoma cell lines T7721 and 7721. METHODS: The human hepatoma cells T7721 which was stably transfected with HAb18G/CD147 and nontransfected 7721 were cultured. The total RNA extracted from T7721 and 7721 was dyeswaplabeled with fluorescein(Cy5 and Cy3) and then analyzed using signal transductionrelated microarray. RTPCR was employed to investigate the differential expression of the pivotal molecule(βigh3) primarily screened by microarray in T7721 and 7721. RESULTS: ① By differential analysis of microarray, we found that the mRNA expression of TGFβinduced molecule βigh3 in T7721 was upregulated significantly, as 4.4 times as that in 7721.Whereas the expression of TGFβ1 mRNA hadnt obvious difference between the two cell lines. ② The results of RTPCR suggested that the expression of βigh3 mRNA in T7721 was much higher than that in 7721 (t=14.42, P<0.01). CONCLUSION: The expression of TGFβinduced molecule βigh3 is positively correlated with HAb18G/CD147, and the overexpression of HAb18G/CD147 promotes the expression of βigh3, which provides a sound basis for further investigating the role of HAb18G/CD147 in invasion and metastasis of human hepatoma cells. ......

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