MnCl2；大鼠；嗜铬细胞瘤PC12细胞；pERK1

锰对PC12细胞生长增殖及对ERK信号转导通路的影响

http://www.100md.com 《第四军医大学学报》 2006年第12期

MnCl2；大鼠；嗜铬细胞瘤PC12细胞；pERK1,2,,MnCl2；大鼠；嗜铬细胞瘤PC12细胞；pERK1,2,0引言,1材料和方

     【Abstract】 AIM: To study the toxicity of different doses of MnCl2 on rat pheochromocytoma PC12 cells in vitro and their effects on signal transduction of extracellular signalregulated kinase 1/2 (ERK1/2). METHODS: PC12 cell cultures were exposed to MnCl2 within the range of 100900 μmol/L. The inhibition of the cells during 7 d was determined by MTT assay and plate clone formation test, and ERK1/2 and pERK1/2 were determined by Western blot. RESULTS: MnCl2 at different concentrations (100, 300, 500, 700 and 900 μmol/L) could inhibit the growth and proliferation of PC12 cells in a dose and timedependent manner and the cell growth curves were changed accordingly (P<0.05). Western blot showed that MnCl2 decreased pERK1/2 expression in a dosedependent manner(P<0.05). CONCLUSION: MnCl2 can significantly inhibit the growth and proliferation of PC12 cells, possibly by downregulating pERK1/2 expression.

    【Keywords】 MnCl2; rats; pheochromocytoma PC12 cell; pERK1/2

    【摘要】目的：研究不同浓度的锰对PC12细胞生长增殖及细胞外信号调节蛋白激酶1/2 (ERK1/2)信号转导通路的影响. 方法：体外培养大鼠嗜铬细胞瘤PC12细胞 ......

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