慢性束缚应激小鼠海马应激性病理效应
束缚应激；海马；天冬氨酸特异性半胱氨酸蛋白酶3；病理形态学,,束缚应激；海马；天冬氨酸特异性半胱氨酸蛋白酶3；病理形态学,【摘要】,【关键词】,1材料与方
【摘要】 目的 观察慢性束缚应激下小鼠海马神经元数目的改变及天冬氨酸特异性半光氨酸蛋白酶3的活性，探讨应激对海马的损害作用及途径。方法 将16只小鼠随机分为对照组和应激组各8只，采用50ml离心管建立慢性束缚应激模型，每晚束缚8min。14d后处死，取小鼠海马常规固定制HE片、免疫组化天冬氨酸特异性半光氨酸蛋白酶3片，在显微镜下进行细胞记数并显微拍照。结果 慢性束缚应激组小鼠海马数目较对照组减少(P<0.05)，同时天冬氨酸特异性半光氨酸蛋白酶3阳性细胞率增加(P<0.05)；镜下神经元发生空泡变性，存在典型凋亡细胞。结论 慢性束缚应激海马神经元凋亡蛋白天冬氨酸特异性半光氨酸蛋白酶3活性增加，天冬氨酸特异性半光氨酸蛋白酶3可能参与了应激状态下海马的细胞凋亡。【关键词】 束缚应激；海马；天冬氨酸特异性半胱氨酸蛋白酶3；病理形态学
Stressful pathological effects of hippocamal in chronic restraint stress mice
Yang Ningbo, Wu Aiqin, Wang Chuansheng, et al
(Department of psychiatry,the first affiliated hospital of Suzhou university,215006,Jiangsu,China)
【Abstract】 Objective To observe changes of hippocamal neurons and activities of Caspase3 in the hippocampus of chronic restraint stress mice and explore the injuring actions and ways of stress to hippocampus. Methods 16 mice were randomly divided into control and stressful group(both n=8),RST devices were constructed with 50ml polypropylene conical tubes, restrain stress was imposed for 8 minutes per night. After Fourteen days mice were killed and brains were taken. The paraffin section for the He and immunohistochemistry Caspase3 were routinely made, counts of positive cell and photomicrographs taken under microscope. Results Decrease of hippocamal neurons were more marked in the stress than in the control group(P<0.05) and rate of positive cell of Caspase3 increased more significantly(P<0.05); Neurons developed vacuolar degeneration and had dead cells. Conclusion Activity of hippocamal Caspase3 increased and Caspase3 might play a role in the death of hippocamal neurons under stress. ......
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