乳腺肿瘤·脂氧合酶抑制剂·端粒_末端转移酶·细胞凋亡·基因

脂氧合酶抑制剂NDGA对乳腺癌MCF7细胞端粒酶和bcl2的影响

http://www.100md.com 《中国普通现代外科进展》 2006年第2期

乳腺肿瘤·脂氧合酶抑制剂·端粒,末端转移酶·细胞凋亡·基因,bcl2,,乳腺肿瘤·脂氧合酶抑制剂·端粒，末端转移酶·细胞凋亡·基因，bcl2,脂氧合酶抑制剂NDGA对乳腺癌MCF7细胞端粒

     【摘要】目的：探讨脂氧合酶抑制剂NDGA对乳腺癌MCF7细胞端粒酶和bcl2表达的影响。方法：将乳腺癌MCF7细胞系培养呈对数生长期，分别以0、1、10、100μmol/L的NDGA处理48h后，RTPCR法检测细胞端粒酶hTERTmRNA的表达水平，流式细胞仪检测细胞bcl2的表达情况。结果：在1、10、100μmol/L的NDGA作用下，3组hTERTmRNA和bcl2的表达均显著低于对照组(P0.001)。结论：NDGA能够下调MCF7细胞端粒酶hTERTmRNA及bcl2的表达，进而抑制细胞增殖，诱导细胞凋亡。

    【关键词】乳腺肿瘤·脂氧合酶抑制剂·端粒，末端转移酶·细胞凋亡·基因，bcl2

    Effects of lipoxygenase inhibitor NDGA on telomerase and bcl2 in breast cancer MCF7 cells

    LIU Huantao,SUN Jingzhong,TANG Lubing

    Department of General Surgery,Qilu Hospital of Shandong University(Jinan 250012,

    China)

    【ABSTRACT】Objective:To explore the effects of lipoxygenase inhibitor NDGA on telomerase and bcl2 of breast cancer MCF7 cells.Methods:MCF7 cells in the logarithmic growth state were selected and measured.Four cell groups (concentration:0,1,10,100μmol/L)were divided in the experiment.After 48h treatment of NDGA,the mRNA expression of telomerase was examined by RTPCR and the expression of bcl2 was measured by flow cytometry.Results:After 48h treatment of different concentration of NDGA,the expressions of hTERTmRNA and bcl2 in NDGA groups were both significantly lower than the control group (P0.001).Conclusion:NDGA can prevent proliferation and induce apoptosis in MCF7 cells by means of the downregulated expressions of hTERTmRNA and bcl2. ......

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