共刺激分子·再灌注损伤·肝移植·大鼠_Wistar_

共刺激分子B71、B72在大鼠热缺血再灌注肝脏表达意义的研究

http://www.100md.com 《中国普通现代外科进展》 2006年第3期

共刺激分子·再灌注损伤·肝移植·大鼠,Wistar,,共刺激分子·再灌注损伤·肝移植·大鼠，Wistar,【摘要】,【关键词】

     【摘要】目的：探讨共刺激分子B71和B72在大鼠肝脏热缺血伤再灌注损伤模型中的表达情况及其免疫学意义。方法：将雄性Wistar大鼠随机分为3组：A组(缺血30min再灌注24h)、B组(缺血60min再灌注24h)、C组(假手术组)。模型制备参考Ohmorid的方法。分别取模型之肝左叶，采用实时反转录聚合酶链反应(PTPCR)检测B71和B72在3组肝左叶组织中mRNA表达情况。结果：B71和B72 mRNA在C组以极低水平表达，而在A组、B组表达却明显增多(P<0.01)，且B组高于A组(P<0.05)。结论：热缺血再灌注之肝脏通过上调B71和B72的表达增加了肝脏的免疫原性。

    【关键词】共刺激分子·再灌注损伤·肝移植·大鼠，Wistar

    Expression of costimulatory molecule B71 and B72 in warm ischemia/reperfusion(I/R) injury of liver in rats

    JI Zhipeng,XU Kesen,LIU Yi,LIU Zhao

    Department of General Surgery,Qilu Hospital of Shandong University(Jinan 250012,China)

    【ABSTRACT】Objective:To investigate the expression of costimulatory molecule B71 and B72 by warm ischemia/reperfusion(I/R)injury in rat liver and the meaning of immunology.Methods:Male Wistar rats were randomly divided into 3 groups:A(ischemia 30 mins,referfusion 24h),B(ischemia 60 mins,referfusion 24h),C(sham operation group).A model of hepatic warm I/R in rats was established according to the method of Ohmorid.The expression of B71 and B72 mRNA was analyzed by realtime reverse transcription polymerase chain reaction(RTPCR).Results:Compared with the sham operation group,the expression of B71 and B72 mRNA increased significantly in the rats undergoing I/R procedure(P<0.01).Conclusion:Warm ischemia/reperfusion injury may increase the immunogenicity of the liver by upregulating the costimulatory molecule B71 and B72. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/07/13/02/81/25.htm

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