脂氧合酶抑制剂NDGA对HT29结肠癌细胞诱导凋亡的研究
脂氧合酶抑制剂·结直肠肿瘤·凋亡,,脂氧合酶抑制剂·结直肠肿瘤·凋亡,1材料与方法,2结果,3讨论,参考文献
【摘要】目的：探讨脂氧合酶抑制剂NDGA在体外对结肠癌HT29细胞株生长抑制、凋亡影响。方法：应用MTT法绘制生长曲线；倒置相差显微镜观察细胞形态变化；扫描电镜观察细胞的超微结构变化，检测凋亡小体；流式细胞仪检测细胞凋亡、细胞周期变化。结果：不同浓度NDGA处理HT29结肠癌细胞，随着药物浓度加大，细胞形态变圆，体积缩小，从瓶壁上逐步脱落，肿瘤细胞凋亡率逐步上升，癌细胞阻滞于G0/G1期。应用扫描电镜可见凋亡小体形成。结论：NDGA对结肠癌HT29细胞具有抑制生长、诱导凋亡作用，凋亡率随药物浓度加大逐步升高。具有剂量依赖效应。【关键词】脂氧合酶抑制剂·结直肠肿瘤·凋亡
Research on apoptosis of colon cancer cell line HT29 induced by NDGA
XIA Guangtao1，ZHANG Yuanchao1 ，WU Sensen2， ZHANG Shangzhong2
1Department of Rheumatism,Shandong Provincial Hospital,Shandong University(Jinan 250021,China)
2Department of Digestion.QiIu Hospital of Shandong University(Jinan250012,China)
【ABSTRACT】Objective:To find out the effect of NDGA (Nordihydroguaiaretic Acid),the lipoxygenase inhibitor,on colon cancer cell line HT29 in vitro from the aspects of cell growth inhibition, cell apoptosis.Methods:We applied respectively.① MTT to draw the growth curve.② inverted phase contrast microscope to observe morphologic change of cells as well as scanning electron microscope to observe changes of cell’s ultramicrostructure and apoptotic body.③ flow cytometry to examine the apoptosis and periodic changes of cells.Results:We used different concentration of NDGA to dispose cancer cell independently.Accompanying the rise in drug’s concentration,apoptosis rate of cells rose gradually,morphologic of cells became round,the volume became small and they abscised from the inner surface of the bottle.apoptotic body could be found through scanning electron microscope.The growth of cells was blocked in the stage of G0/G1.Conclusions:NDGA can inhibit colon cancer cell line HT29 growth and induce its apoptosis,the rate of which rises following the increase of drug concentration and displays the relying effect of doses.The drug blocks the cell in the stage of G0/G1. ......
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