胃肿瘤·转录因子·寡脱氧核苷酸类_反义·新生血管化

胃癌组织中E26转录因子1表达与血管生成的相关性研究

http://www.100md.com 《中国普通现代外科进展》 2006年第2期

胃肿瘤·转录因子·寡脱氧核苷酸类,反义·新生血管化,病理性,,胃肿瘤·转录因子·寡脱氧核苷酸类，反义·新生血管化，病理性,1材料与方法,2结

     【摘要】目的：研究胃癌组织中E26转录因子1(Ets1)表达与血管生成的关系。方法：应用免疫组织化学方法(SP法)检测86例胃癌标本中Ets1的表达及微血管密度(MVD)；建立裸鼠胃癌皮下种植瘤模型，分别应用Ets1反义，正义寡核苷酸与PBS行瘤内注射，应用逆转录聚合酶链式反应检测瘤组织Ets1mRNA的变化，并检测瘤组织Ets1蛋白及微血管密度的差异。结果：胃癌组织中Ets1标记指数(LI)和MVD呈正相关(γ=0.495,P<0.05)；经Ets1反义寡核苷酸治疗后，裸鼠瘤组织Ets1mRNA及Ets1蛋白表达降低，反义组MVD显著低于正义组与PBS组(10.4±3.1 VS 24.5±8.4,20.6±7.5,P<0.01)。结论：胃癌组织中Ets1在促进血管生成中起重要作用。

    【关键词】胃肿瘤·转录因子·寡脱氧核苷酸类，反义·新生血管化，病理性

    Correlation of Ets1 expression and angiogenesis in gastric cancer

    DING Yinlu1,GONG Xiangqian2,LI Xia1,FU Qinye1,ZHANG Jianliang1,LI Zhaoting3

    1Department of General Surgery,the Second Hospital of Shandong University(Jinan

    250033,China)

    2Department of General Surgery,Yuhuangding Hospital of Qingdao University(Yantai

    264000,China)

    3Department of General Surgery,Qilu Hospital of Shandong University(Jinan 250012,

    China)

    【ABSTRACT】Objective:To investigate the relationship of the expression of Ets1 and angiogenesis of gastric cancinoma.Methods:Expression of Ets1 and microvessel density(MVD)was detected immunohistochemically in paraffin imbedded specimens of 86 cases of gastric cancinoma.Human gastric carcinoma transplanted subcutaneously in nude mice model was constructed,and subquently the mice were devided into three groups:control group,sense oligoxydeonucleotide(sODN)group and antisense oligoxydeonucleotide(AsODN) group:After treatment respectively,changes of Ets1mRNA of transplanted tumor were detected by the reverse transcription polymerase chain reaction method(RTPCR),Ets1 protein and MVD was examined immunohistochemically.Results:A positive correlation was found between the expression of Ets1 and MVD in human gastric carcinoma tissues.Ets1 AsODN could underregulate the expression of Ets1mRNA and Ets1 protein.MVD of AsODN group was signicantly lower than control group and sODN group(P<0.01).Conclusion:Ets1 may promote angiogenesis of gastric cancinoma. ......

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