反相高效液相色谱法测定山楂中的有机酸
反相高效液相色谱,山楂,有机酸,,反相高效液相色谱，山楂，有机酸,摘要,关键词,1引言,2实验部分,3结果与讨论,References
摘 要 采用Hypersil ODS2(4.6 mm×250 mm，5 μm)色谱柱，以磷酸盐缓冲溶液(pH=2.6)为流动相，流速0.8 mL/min，检测波长210 nm，建立了山楂中柠檬酸、苹果酸等有机酸的高效液相色谱分析方法。其中柠檬酸和苹果酸的线性范围分别为0.50～50.04 μg、0.10～10.00 μg；3个浓度水平的回收率分别为95.5%～966%、100.2%～103.7%，RSD分别为0.11%～0.38%、0.41%～2.53%；检出限分别为0.04 μg、0.03 μg(S/N=3)。测定了5种不同产地山楂果肉及山楂核中的柠檬酸、苹果酸的含量。所建立的方法快速﹑灵敏度高、重复性好，可用于山楂中有机酸的测定。关键词 反相高效液相色谱，山楂，有机酸
Analysis of Organic Acids in Hawthorn by Reversed Phase High Performance Liquid Chromatography
Jin Gaowa， Zhang Feifang*， Xue Xingya， Xu Qing， Liang Xinmiao
(Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023)
Abstract Hawthorn is a traditional Chinese medicine and fruit. It contains numerous organic acids. It is reported that organic acids have the ability to cure indigestion and have many other functions. A quantitative reversedphase high performance liquid chromatography method using diode array detector has been developed for the determination of citric acid and malic acid in hawthorn. A Hypersil ODS2 column was used with phosphate buffer solution (pH=2.6) as the mobile phase at a flow rate of 0.8 mL/min. The column temperature was 25℃ and the UV detection wavelength was 210 nm. The calibration curve for citric acid and malic acid was in good linearity in the range of 0.50-50.04 μg with the regression equation Y=78.2X-0.08 ......
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