白介素2·白介素4·肝炎_乙型·多态性_单核苷酸_

中国北方两市部分人群IL2、IL4基因多态性与乙肝关联性的研究

http://www.100md.com 《中国普通现代外科进展》 2006年第3期

白介素2·白介素4·肝炎,乙型·多态性,单核苷酸,,白介素2·白介素4·肝炎，乙型·多态性，单核苷酸,【摘要】,【关键词】,1

     【摘要】目的：探讨北方地区人群IL2、IL4基因SNPs与乙肝发生的关联。方法：提取中国北方两市乙肝患者及健康对照外周血基因组DNA，用PCR方法扩增IL2基因包含突变位点330的片段以及IL4基因包含突变位点590的片段，采用RFLP明确健康个体乙肝患者基因型。采用PHASE10软件构建这3个多态性位点的个体单倍体型。以非条件Logitic回归校正混杂因素，并进行多态性与乙肝风险关联性的统计学分析。结果：2个多态性位点在北方两市部分人群中具有多态性。两组人群中330(IL2)多态性位点差异有统计学意义(P<0.05)；590(IL4)多态性位点差异无统计学意义(P>0.05)。这两个位点所构建的单倍体型中，含有GT单倍体的个体在两组人群中具有高度差异(P<0.01)。结论：IL2基因330位点的多态性对于乙肝的发生有着显著的易患关联，其杂合、纯合形式分别增加乙肝患病风险1.67倍和3.81倍。具有GT单倍体的个体对于乙肝的发生同样具有易感关联，其增加乙肝患病风险约为1.94倍。

    【关键词】白介素2·白介素4·肝炎，乙型·多态性，单核苷酸

    The association of IL2 and IL4 polymorphisms and hepatitis B in population of north China

    WANG Kun1,SHI Changwen1,GAO Yong1,YU Zhenhai1,NING Tao2,CAO Bangwei2,LI Yong2

    1Department of General Surgery,Qianfoshan Hospitical of Shandong Privince(Jinan

    250014,China)

    2Department of Genetics,Beijing Institute for cancer Research(Beijing 100036,China)

    【ABSTRACT】Objective:To investigate the association between the single nucleotide polymorphisms(SNPs) of transporter associated with interleukin2(IL2),interleukin4(IL4)and the susceptibility of hepatitis B in north China.Methods:Genomic DNA was extracted from the peripheral blood leukocytes of hepatitis B patients and healthy controls.Two fragments covering 330 of the IL2 gene and 590 of the IL4 gene were amplified by polymerase chain reaction (PCR).The SNPs were revealed by restriction fragment length polymorphism (RELP).Software PHASE 1.0was used to construct the haplotype of every individual.Unconditional Logitic regression model was used to analyze the statistical association of genotype or haplotype in two groups adjusted by gender and age.Results:There was significant difference in the SNPs of IL2 between the healthy controls and the heptitis B patients in north China.Significant difference was also found in the combination of /GT to the two groups.Conclusion:SNPs of IL2(330) may have relation to the susceptibility o heptitis B./GT was also related to the susceptibility of heptitis B.These findings suggest that SNPs of IL2 is one of the important host factors to the infection of the heptitis B. ......

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