方剂槲芪散及君药槲寄生提取物对人肝癌细胞生长的抑制作用
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李 霞, 丰 平, 文朝阳, 朱 淼, 刘树红,
槲芪散;槲寄生提取物;细胞周期;细胞凋亡;活性氧李霞,丰平,文朝阳,朱淼,刘树红,王学江.方剂槲芪散及君药槲寄生提取物对人肝癌细胞生长的抑制作用. 世界华人消化杂志2006;14(20)1
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李霞, 丰平, 刘树红, 王学江, 首都医科大学病理生理学教研室 北京市 100069
文朝阳, 首都医科大学生物化学教研室 北京市 100069
朱淼, 首都医科大学组织与胚胎学教研室 北京市 100069
李霞, 2004年首都医科大学硕士, 主要从事肝癌前病变的细胞分子生物学研究.
北京市中医药科技项目, No. JJ2005-10
北京市教育委员会科技发展项目, No. M200610025003
通讯作者: 王学江, 100069, 北京市右安门外西头条10号, 首都医科大学病理生理学教研室. xj-w@163.com
电话: 010-83911434 传真: 010-83911484
收稿日期: 2006-04-03 接受日期: 2006-05-22
IGrowth inhibition of human hepatocarcinoma cells induced by Chinese herbs Huqi San and its principal drug mistletoe extracts
Xia Li, Ping Feng, Zhao-Yang Wen, Miao Zhu, Shu-Hong Liu, Xue-Jiang Wang
Xia Li, Ping Feng, Shu-Hong Liu, Xue-Jiang Wang, Department of Pathophysiology, Capital University of Medical Sciences, Beijing 100069, China
Zhao-Yang Wen, Department of Biochemistry, Capital University of Medical Sciences, Beijing 100069, China
Miao Zhu, Department of Histology and Embryology, Capital University of Medical Sciences, Beijing 100069, China
Supported by the Traditional Chinese Medicine Technology Project of Beijing City, No. JJ2005-10, and Technology Development Program of Beijing Education committee, No. M200610025003
Correspondence to: Dr. Xue-Jiang Wang, Department of Pathophysiology, Capital University of Medical Sciences, 10 Youanmenwai Xitoutiao, Beijing 100069,China. xj-w@163.com
Received: 2006-04-03Accepted: 2006-05-22
AbstractAIM:To probe the effect of Chinese herbs, Huqi San (HQS) and its principal drug mistletoe extracts on the growth of human hepatocar-cinoma cell line SMMC-7721 and the levels of intracellular reactive oxygen species (ROS).
METHODS:The proliferation of SMMC-7721 cells inhibited by HQS (0.3125, 0.625, 1.25 g/L), mistletoe polysaccharides (0.625, 1.25, 2.5, 5 g/L) and total alkaloid of mistletoe (3, 6, 12 g/L) at 48, 72 and 96 h were observed by MTT assay. The cell cycle and apoptosis rate of SMMC-7721 cells were examined by flow cytometry ......
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