【摘要】目的：观察AngⅡ对体外培养的人脐静脉内皮细胞(HUVECs)表达内皮型一氧化氮合酶(eNOS)及内皮NO生成的影响，探讨其可能机制。 方法： 采用胰蛋白酶消化法进行HUVECs的原代培养，3～5代用于实验；通过RT-PCR和Western Blot检测eNOS的mRNA和蛋白表达水平；利用Griees反应原理测定上清NO释放量。结果：与无刺激组相比， AngⅡ(10-8 M、10-7 M、10-6M)抑制HUVECs eNOS mRNA和蛋白表达(P<0.05)；HUVECs未受刺激时NO生成量是(50.21±4.39)μM，不同浓度的AngⅡ(10-8 M、10-7 M、10-6M)与HUVECs共孵育12 h可明显减少NO 释放(30.01±4.10)μM、(21.33±4.81)μM、(16.35±2.56)μM，使其浓度分别下降49.7%、63.4%、71.1%。结论：AngⅡ明显抑制HUVECs表达eNOS，并呈浓度效应减少内皮NO的释放。

    【关键词 】 血管紧张素Ⅱ；内皮型一氧化氮合酶；一氧化氮

    Ang Ⅱ reduces eNOS expression and decreases the production of NO in HUVECs

    XU Ying-hua, DING Yue-xia, LIU Shi-ming，et al

    (GuangDong Province Rongjun Hospital, Guangzhou 510260 China; Department of Cardiology of the Second Affiliated Hospital of Guangzhou Medical College)

    【Abstract】Objective：To observe the affection of AngII to eNOS expression and the production of NO in HUVECs. Methods:Human umbilical vein was isolated and culcured. Passage 3~5 of cultured HUVECs were incubated with 10-9 M 、10-6 M for 12 h.Total RNA was extracted, and the expression of eNOS mRNA or protein was assessed by RT-PCR or Western Blot; While NO production was measured by Griees (nitrate reductase method).Results: with AngII-stimulated in different concentration (10-8、10-7、10-6 M)for 12 h, eNOS was down-regulated.Conclusions: AngⅡ could inhabit the expressions of eNOS on dose dependant both RNA and protein. Another also decreases NO production on dose. ......