白细胞介素2；白细胞介素18；自然杀伤细胞；骨髓瘤细胞

白细胞介素2协同白细胞介素18活化自然杀伤细胞活性的作用

http://www.100md.com 《中华首席医学网》 2006年第3期

白细胞介素2；白细胞介素18；自然杀伤细胞；骨髓瘤细胞,,白细胞介素2；白细胞介素18；自然杀伤细胞；骨髓瘤细胞,摘要,关键

     摘要：目的研究白细胞介素2(IL2)和白细胞介素18(IL18)诱导自然杀伤(NK)细胞活化和抗骨髓瘤细胞活性的协同作用。方法流式细胞仪方法检测NK细胞表面CD69的表达；阴性细胞纯化法用于获得高纯度的NK细胞; ELISA测定NK细胞培养上清液中干扰素(IFNγ)的浓度；标准铬51释放试验评估NK细胞杀伤骨髓瘤细胞的能力。结果与单一应用IL18相比，低浓度IL2和IL18联合应用显著提高了NK细胞的活性、分泌干扰素(IFNγ)的水平和杀伤骨髓瘤细胞的作用。结论低浓度IL2具有协同IL18活化NK细胞抗骨髓瘤细胞的作用，此结果为IL2和IL18联合应用治疗骨髓瘤奠定了一定的理论基础。

     关键词：白细胞介素2；白细胞介素18；自然杀伤细胞；骨髓瘤细胞

    The effect of interleukin2 (IL2) synergized with IL18 to enhance activities of natural killer cells

    Song Ping, Zheng Chengyun

    (Clinical Examination Department, Xian Health School, Xian 710054;the Second Hospital of Shandong University, Jinan 25000, China)

    ABSTRACT: Objective To explore potential synergetic effects of interleukin2 (IL2) with IL18 on induction of natural killer (NK) cell activity against myeloma cells in vitro. Methods CD69 expression on NK cells were defined by Flow Cytometry; IFN γ level in supernatants of NK cell culture was measured by ELISA; pure NK cell population was obtained from peripheral blood by using cell purification kit; standard Cr51 release method was used to test NK cell cytotoxicity against myeloma cells in vitro. Results The combined application of IL18 with low dose of IL2 (10u/mL) significantly enhanced NK cell activation, IFNγ secretion and NK cell cytolytic activity against myeloma cells (U266 and RPMI8226) as compared to IL18 alone, respectively. Conclusion IL2 synergized with IL18 to induce NK cells activates antimyeloma cell in vitro and combined use of IL18 and low dose IL2 could be an alterative immunotherapy approach for cancers including myeloma. ......

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