纤维环细胞；硅橡胶膜；逆转录聚合酶链式反应；流式细胞术

种植于硅橡胶膜上的大鼠纤维环细胞的表型特征研究

http://www.100md.com 《第四军医大学学报》 2006年第13期

纤维环细胞；硅橡胶膜；逆转录聚合酶链式反应；流式细胞术,,纤维环细胞；硅橡胶膜；逆转录聚合酶链式反应；流式细胞术,0引言,1材料和方法,2结果

     Phenotypic characteristics of rat annulus fibrosus cells cultured on flexible silicone membranes

    GUO ZhiLiang, ZHOU Yue, CHENG Min, CAO GuoYong, LI HuaZhuang, TENG HaiJun

    Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China

    【Abstract】 AIM: To compare the phenotypic characteristics of rat annulus fibrosus (AF) cells cultured on flexible silicone membranes with the same cells cultured in plastic plates. METHODS: The morphology of AF cells cultured in different substrates was observed under an inverted phasecontrast microscope and a transmission electron microscope. Proteoglycan was stained with toluidine blue for observing its expression. The contents of collagen typeⅠ, Ⅱand aggrecan mRNA were determined by RTPCR. The expression of integrin β1 was monitored by flow cytometry. Cell adhesion to silicone membranes was also measured. RESULTS: The AF cells cultured in different substrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on flexible silicone membranes and in plastic plates. The cells growing in different substrates expressed the same levels of collagen typeⅠ, Ⅱ and aggrecan mRNA and integrin β1. AF cells grew well on silicone membranes. CONCLUSION: AF cells cultured on flexible silicone membranes maintain the stability of phenotype and may be appropriate for further studying the metabolic responses to mechanical stimuli at the cellular level. ......

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