Establishment of a method of deterª²mining MN9202 in bioª²medium and its application

    RAN Yuª²Hua, ZHOU Siª²Yuan, LIU Xinª²You, TENG Zengª²Hui, YANG Runª²Tao, YANG Zhiª²Fu, MEI Qiª²Bing

    Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To establish a method to determine 2,6ª²Dimethylª²4ª²(3ª²nitropheny1)ª²1,4ª² dihydropyridineª²3ª²carboxylic acid dimethylesterª²5ª²carboxylic acid pentyl ester (MN9202) in biological medium by reverseª²phase highª²performance liquid chromatography (RTª²HPLC). METHODS: The specimen containing the lysate of Cacoª²2 cells and rat plasma were extracted with ether. The organic phase was removed into the tubes and then evaporated in ventilation cabinet. The residue was dissolved in 50 ¦ÌL of methanol, then 20 ¦ÌL solution was drawn and detected by RTª²HPLC. RESULTS: MN9202 could be disª²associated from interference in the certain mobile phrases, the regression equation of the standard curve to detect the cell samples was Y=0.0048X+0.0592, r=0.9997, n=7 (P<0.01), the linear range was 20-500 mg/L; The standard curve to detect MN9202 in the plasma was worked out, and the regression equation was Y=0.0058X+0.1468, r=0.9998, n=7 (P<0.01), the linear range was 20-500 mg/L. CONCLUSION: The analytical method was convenient, stable, sensitive and accurate. It could be applied to the determination of MN9202 in biological medium. ......