Effects of exogenous ILª²18 gene on the proliferation and related gene expression in C6 glioma cells

    JIANG Changª²Wen, YAN Yunª²Li, MA Weiª²Dong, GAO Qingª²Hua, SHEN Yuan, ZHENG Liª²Fen, WANG Yanª²Ling, ZHOU Naª²Jing

    Cell Biology Division, School of Basic Medicine, 2Department of Epidemiology, School of Public Health, Hebei Medical University, Shijiazhuang 050017, China

    ¡¾Abstract¡¿ AIM: To investigate the effects of exogenous ILª²18 gene on the proliferative ability and related gene expression in transfected C6 glioma cells. METHODS: The cell cycle distributions and proliferation index of C6/ILª²18 and its parental C6 cells were detected by flow cytometry. RTª²PCR, immunocytochemistry and Western blot analysis were applied to test the mRNA and protein expression of cyclin D1, cyclin B1 and Bclª²2 in C6/ILª²18 and C6 cells respectively. RESULTS: Compared with C6 cells, C6/ILª²18 cells were arrested at G0/G1 phase with fewer G2/M cells. The proliferation index of C6/ILª²18 cells was lower than that of C6 cells. At both mRNA and protein levels, the Bclª²2, cyclin B1 and cyclin D1 expression in C6/ILª²18 cells were decreased. CONCLUSION: Exogenous ILª²18 gene could reduce the proliferative ability of C6 cells by inhibiting the progress of cell cycle and downª²regulating the Bclª²2, cyclin B1 and cyclin D1 expressions. ......