组织芯片和常规制片检测乳腺癌相关癌基因产物的比较
乳腺肿瘤；组织芯片；免疫组织化学；癌基因,,],乳腺肿瘤；组织芯片；免疫组织化学；癌基因,1材料与方法,2结果,3讨论,参考文献：
[摘 要] 目的：探讨不同规格组织芯片在乳腺癌相关基因产物检测中的应用价值。方法：采用微波修复免疫组织化学SP法和组织芯片技术，检测ER、PR、nm23、Her2、p53在31例乳腺癌组织中的表达，并与常规病理学方法进行比较分析。结果：不同规格组织芯相比较，免疫组化结果几乎完全一致。常规制片免疫组化阴性，而组织芯片免疫组化阳性者包括ER2例、PR1例；常规制片免疫组化阳性，而组织芯片免疫组化阴性者包括nm23、Her2各1例。部分病例常规制片免疫组化和组织芯片免疫组化阳性表达强度略有不同。结论：组织芯片的免疫组化结果与常规制片免疫组化比较，经统计学处理，差异无显著性(P>0.05)。组织芯片技术有良好应用价值和广阔发展前景。[关键词] 乳腺肿瘤；组织芯片；免疫组织化学；癌基因
Comparative Analysis on the Expression of Associated Genes Production in Breast
Carcinoma by Tissue Microarray and by Traditional Pathological Investigation
JIANG Hui1,ZHOU Xiaoqiu2(1.Shenzhen Maternal and Child Health Institute,Shenzhen，Guangdong 518028,China;2.Dongying People's Hospital,Dongying,Shandong 257091,China)
Abstract:Objective To exploethe significance of each type of tissue microarrary on the examine of associated genes production in breast carcinoma.Methods Tissue microarray technology and immunohistochemistry were used to detect the expression of ER,PR,nm23,Her2 and p53 in 31 cases of breast carcinoma and compared theresult with the traditional pathological investigation.Results The difference was no found detween the immunohistochemistric result by 1.0 mm tissue microarray and by 0.5 mm tissue microarray.2 cases of ER and 1 of PR were negative by traditional pathological technology,but were positive by tissue microarray.1 case of nm23 and 1 of Her2 were positive by traditional pathological technology,but were negative by tissue microarray.On the expressional strength of ER,PR,nm23,Her2 and p53,the nigligible difference was found detween by traditional pathological technology and by tissue microarray in some cases.Conclusion On the positive rate of ER,PR,nm23,Her2 and p53,the significant difference was no found detween the result with the traditional pathological investigation and with the tissue microarray(P>0.05).It has important pracyical significance and broad application prospect in pathology. ......
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