菊花脑保肝作用的有效部位筛选
菊花脑；有效部位；CCl4；肝损伤,,菊花脑；有效部位；CCl4；肝损伤,1材料,2方法,3结果,4讨论,【参考文献】
【摘要】 目的 在菊花脑不同极性提取部位(CFDN)中筛选出保肝作用的有效部位。方法 采用0.1%CCl4(10ml/kg)ip小鼠，造成急性肝损伤的模型，测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)，观察肝组织病变；用CCl4损伤大鼠肝细胞，MTT法测定肝细胞增殖活性。结果 CFDN的60%乙醇和95%乙醇柱洗脱部位能显著降低小鼠CCl4肝损伤血清中ALT和AST的活性(P<0.01，P<0.05)，病理学观察显示：该部位能明显减轻肝损伤的程度(P<0.01，P<0.05)，CFDN的60%乙醇和95%乙醇柱洗脱部位能显著提高肝细胞活性，促进肝细胞增殖(P<0.01)。结论 CFDN的60%乙醇和95%乙醇柱洗脱部位具有显著的保肝作用。【关键词】 菊花脑；有效部位；CCl4；肝损伤
Screening of the active fractions from Chrysanthemum nankingense on their liver-protective effects
REN Ai-nong,LIU Chun-yu,YANG Nian-yun.Jiangsu Provincial Institute of Traditional Chinese Medicine,Nanjing,Jiangsu Province Engineering Research Center For Pharmaceutial,Nanjing 210028,China
【Abstract】 Objective To screen the active fractions from Dendranthema Nankingense of Chrysanthemum nankingense on their liver-protective effects.Methods The mouse models of carbon tetrachloride-induced acute hepatic injury were employed in this study.The activity of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in the serum were assaied.Also pathological changes were observed in liver tissues.The proliferous activities were assaied by MTT in cells of liver-injured rat by 0.1%CCl4(10ml/kg)ip.Results The CFDN of 60% alchohol and 95% alchohol can debase ALT and AST activities in the serum of liver-injuried mice(P<0.01 ......
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