重组腺病毒Ad-IkBaM在5-氟尿嘧啶诱导胃癌细胞凋亡中的作用
http://www.100md.com
胡丽红, 刘冰熔, 刘 丹, 关景明, 吕志武,
核转录因子kB;胃癌;5-氟尿嘧啶;基因治疗,胡丽红,刘冰熔,刘丹,关景明,吕志武,杜雅菊,通讯作者,Roleofrecombinan
 |
| 第1页 |
参见附件(926KB，5页)。
胡丽红, 刘冰熔, 刘丹, 关景明, 吕志武, 杜雅菊, 哈尔滨医科大学附属第二医院消化科 黑龙江省哈尔滨市 150086
胡丽红, 哈尔滨医科大学附属第二医院2003年消化内科硕士生.
黑龙江省攻关重点资助项目, No. GC02C148-01
2005年哈尔滨医科大学优秀研究生创新基金资助项目, No. HCXS2005015
通讯作者: 刘冰熔, 150086, 黑龙江省哈尔滨市, 哈尔滨医科大学附属第二医院消化科. liubingrong@medmail.com.cn
电话: 0451-86605980 传真: 0451-86605980
收稿日期: 2006-03-25 接受日期: 2006-05-08
Role of recombinant adenovirus IkBaM in 5-fluorouracil-induced apoptosis of gastric carcinoma SGC-7901 cells
Li-Hong Hu, Bing-Rong Liu, Dan Liu, Jing-Ming Guan, Zhi-Wu Lv, Ya-Ju Du
Li-Hong Hu, Bing-Rong Liu, Dan Liu, Jing-Ming Guan, Zhi-Wu Lv, Ya-Ju Du, Department of Digestive Diseases, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Supported by the Key Development Program of Heilongjiang Province, No. GC02C148-01, and Innovation Fund for Excellent Graduates of Harbin Medical University, No. HCXS2005015
Correspondence to: Dr. Bing-Rong Liu, Department of Digestive Diseases, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China. liubingrong@medmail.com.cn
Received: 2006-03-25 Accepted: 2006-05-08
Abstract
AIM: To investigate the role of recombinant adenovirus IkBaM (Ad-IkBaM) on the apoptosis of gastric carcinoma SGC-7901 cells induced by 5-fluorouracil (5-FU) as well as the possible mechanism.
METHODS: The cultured SGC-7901 cells were divided into group A, B and C. The cells in group A and B were infected with Ad-IkBaM and Ad-IkBa, respectively, and those in group C served as controls. 5-FU was added to each group at the concentration of 5 mg/L, respectively. Electrophoretic mobility shift assays were used to detect the activation of nuclear facter kappa B (NF-kB) in all the groups, and the 5-FU-induced apoptosis of SGC-7901 cells was tested by MTT and TUNEL method.
RESULTS: After 5-FU treatment, NF-kB was activated in gastric carcinoma cells, but in the cells infected with Ad-IkBaM, the activity of NF-kB was inhibited ......
您现在查看是摘要介绍页，详见PDF附件(926KB，5页)。