原癌基因pim-3的克隆及其对肝癌细胞凋亡的影响
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邓 欢, 刘亮明, 张吉翔, 罗 杰, 尹 东,
肝癌;原癌基因pim-3;克隆;凋亡;荧光显微镜;MTT比色试验;流式细胞术,邓欢,刘亮明,张吉翔,罗杰,熊瑛,汤蕾,谢正元,尹东,邓欢,通讯作者,C
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邓欢, 刘亮明, 张吉翔, 罗杰, 熊瑛, 汤蕾, 谢正元, 南昌大学第二附属医院消化内科 江西省分子医学重点实验室 江西省南昌市 330006
尹东, 江西省分子医学重点实验室 江西省南昌市 330006
邓欢, 2004年南昌大学医学院本科毕业, 现为南昌大学第二附属医院2004级在读硕士研究生, 主要从事消化系肿瘤基因治疗的基础和临床研究.
国家自然科学基金项目, No. 30160032, No. 30360037
江西省科委重点项目, No.200110300101, No.20041B0300300
通讯作者: 张吉翔, 330006, 江西省南昌市民德路1号, 南昌大学第二附属医院消化内科、江西省分子医学重点实验室. jixiangz@tom.com
电话: 0791-6292706 传真: 0791-6262262
收稿日期: 2006-05-30 接受日期: 2006-06-24
Cloning of pro-oncogenepim-3 cDNA and its effect on apoptosis of hepatocellular carcinoma cells
Huan Deng, Liang-Ming Liu, Ji-Xiang Zhang, Jie Luo, Dong Yin, Ying Xiong, Lei Tang, Zheng-Yuan Xie
Huan Deng, Liang-Ming Liu, Ji-Xiang Zhang, Jie Luo, Ying Xiong, Lei Tang, Zheng-Yuan Xie,Department of Gastroenterology, the Second Affiliated Hospital of Nanchang University, Jiangxi Provincial Key Laboratory of Molecular Medicine, Nanchang 330006, Jiangxi Province, China
Dong Yin,Jiangxi Provincial Key Laboratory of Molecular Medicine, Nanchang 330006, Jiangxi Province, China
Supported byNational Natural Science Foundation of China, No. 30160032 and No. 30360037; and the Key Project of Science and Technology Committee of Jiangxi Province, China, No. 200110300101 and No. 20041B0300300
Correspondence to:Ji-Xiang Zhang, Department of Gastroenterology, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China. jixiangz@tom.com
Received:2006-05-30 Accepted:2006-06-24
Abstract
AIM: To clone rat pim-3 cDNA and construct its eukaryotic expressing plasmid, and to investi-gate the expression of pim-3 and its effect on the apoptosis in SMMC-7721 cells.
METHODS: Total RNA was isolated from rat skeletal muscle tissues with TRIzol method. pim-3 cDNA was obtained by reverse transcription-polymerase chain reaction (RT-PCR) and constructed into eukaryotic expression vector pEGFP-N2, which expressed green fluorescent protein ......
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