【摘要】 目的 观察战创伤引起的关节缺血再灌注损伤(ischemia reperfusion，IR)过程中滑膜及软骨形态、结构和功能变化，为明确IR的机制及防止方法提供依据。方法 在建立肢体缺血再灌注模型的基础上，利用中药进行干预，并观察滑膜及软骨组织形态、代谢及功能变化。结果 IR初期，关节滑膜及软骨组织中丙二醛(MDA)、乳酸、多型核白细胞(PMN)明显增高，而超氧化物歧化酶(SOD)及软骨中Ⅱ型胶原降低；应用中药治疗组上述指标差异具有统计学意义(P<0.05)，而且修复时间明显缩短，功能明显改善。结论 IR确可致关节滑膜及软骨损伤，且是发生继发创伤性关节炎的重要因素。应用自制的中药消灵仙后可减轻病理生理变化、组织病理改变，减轻关节病损，改善关节功能。

    【关键词】 缺血再灌注损伤；滑膜；软骨；黄芪；红花

    The effects of Chinese medicine xiaolingxian ointment on the ischemia-reperfusion injury in rabbits knee

    WANG Cheng-qi，WANG Jian-li，SUN Xin-jun，et al.The 89th Hospital of PLA，The Institute of Orthopaedic Trauma of PLA，Weifang，Shandong 261021，China

    【Abstract】 Objective To investigate the effects of Chinese medicine xiaolingxian ointment on the ischemia-reperfusion in rabbits knee and offer experimental proofs to prevent or to treat the dysfunction of the joints after replantation in digital or limb amputation clinically.Methods 50 New Zealand health white rabbits，eithe male or female，weighting (2.88±0.38)kg，were randomized into two groups，ischemia reperfusion group(A)，xiaolingxian ointment group(B). 25 rabbits were devided into five sub-groups randomly in each group. Rabbits were sacrificed at 1、2、4、8and 12 weeks after ischemia-reperfusion. Synovia and synovial membrane were gathered to be measured by lactic acid、SOD、MDA、NOS and observed by light microscope、electron microscope and immunochemical method.Results The surface of synovium was destructed accompanied with degeneration and necrosis of the synoviocytes. In group B ......