反义肝素酶基因对胰腺癌细胞体外增殖和侵袭的抑制作用
http://www.100md.com
杨 彦, 崔 明, 陈 陵, 段体德
肝素酶;反义;胰腺癌;转染;增殖;侵袭,杨彦,崔明,陈陵,段体德,杨彦,通讯作者,Inhibitoryeffectsofantisencehapar
 |
| 第5页 |
 |
| 第1页 |
参见附件(1043KB，6页)。
杨彦, 崔明, 成都军区昆明总医院普通外科 云南省昆明市 650032
陈陵, 第三军医大学西南医院消化专科中心 重庆市 400038
段体德, 昆明医学院第一附属医院普通外科 云南省昆明市 650032
杨彦, 博士, 主治医师, 主要从事消化道肿瘤方面的工作和研究.
通讯作者: 段体德, 650032, 云南省昆明市, 昆明医学院第一附属医院普通外科. tdduan@163.com
电话: 0871-5324888-2442
收稿日期: 2006-04-11 接受日期: 2006-05-26
Inhibitory effects of antisence haparanase gene on proliferation and invasion of human pancreatic cancer cell line SW1990 in vitro
Yan Yang, Ming Cui, Ling Chen, Ti-De Duan
Yan Yang, Ming Cui,Department of General Surgery, Kunming General Hospital of Chendu Military Command, Kunming 650032, Yunnan Province, China
Ling Chen,Department of Gastroenterology, Southwest Hospital of the Third Military Medical University, Chongqing 400038, China
Ti-De Duan,Department of General Surgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
Correspondence to:Ti-De Duan, Department of General Surgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China. tdduan@163.com
Received:2006-04-11 Accepted:2006-05-26
Abstract
AIM: To investigate the inhibitory effects of antisense heparanase gene on the proliferation and invasion of human pancreatic cancer cell line SW1990 in vitro.
METHODS: Human pancreatic cancer cell line SW1990 was transfected with the plasmid expressing antisense heparanase gene. Meanwhile, the empty vector and non-transfection group were designed. The cell cycle distribution was analyzed by flow cytometry; the protein expression of heparanase gene was detected by Western blot and immunohistochemistry, and the mRNA transcription level was assayed by reverse transcription-polymerase chain reaction (RT-PCR). The colony-forming unit assay was used to measure the ability of cell growth, and Transwell chamber model was employed to test the ability of cell invasion in vitro ......
您现在查看是摘要介绍页，详见PDF附件(1043KB，6页)。