PGE2对胃癌MKN28细胞VEGF表达的影响
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肖炜明, 施瑞华, 丁岩冰, 吴 健, 童建东,
前列腺素E2;血管内皮生长因子;mRNA转录;蛋白表达,PGE2对胃癌MKN28细胞VEGF表达的影响,肖炜明,施瑞华,肖炜明,丁岩冰,吴健,童建东,严志刚,通讯作者:,Effectsofpr
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肖炜明, 施瑞华, 南京医科大学附属第一医院消化科 江苏省南京市 210029
肖炜明, 丁岩冰, 吴健, 童建东, 严志刚, 扬州大学第二临床医学院 江苏省扬州市 225001
江苏省卫生厅135工程重点人才基金, No. RC2002053
通讯作者: 施瑞华, 210029, 江苏省南京市, 南京医科大学第一附属医院消化科. ruihuashi@126.com
电话: 025-83674636
收稿日期: 2006-07-10 接受日期: 2006-07-31
Effects of prostaglandin E2 on expression of vascular endothelial growth factor in human gastric cancer cell line MKN28
Wei-Ming Xiao, Rui-Hua Shi, Yan-Bing Ding, Jian Wu, Jian-Dong Tong, Zhi-Gang Yan
Wei-Ming Xiao, Rui-Hua Shi,Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Wei-Ming Xiao, Yan-Bing Ding, Jian Wu, Jian-Dong Tong, Zhi-Gang Yan,the Second Clinical Medical Collage of Yangzhou University, Yangzhou 225001, Jiangsu Province, China
Supported bythe Key Talent Fund from ‘135’ Project of Health Department of Jiangsu Province, No. RC2002053
Correspondence to:Rui-Hua Shi, Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. ruihuashi@126.com
Received:2006-07-10 Accepted:2006-07-31
Abstract
AIM: To investigate the expression of vascular endothelial growth factor (VEGF) in human gastric cell line MKN28 in vitro, and identify whether exogenous prostaglandin E2 (PGE2) can increase the expression of VEGF.
METHODS: Human gastric cell line MKN28 was culture in vitro and then treated with 0.1, 1, 5, and 10 μmol/L PGE2 for 3 h. Real-time polymerase chain reaction (PCR) and Western blot were used to detect the expression of VEGF mRNA and protein, respectively.
RESULTS: The transcription of VEGF mRNA increased in a dose-dependent manner after PGE2 treatment, and the difference between the groups of 0.1, 1, 5, 10 μmol/L PGE2 treatment and the control group had statistical significances (0 ......
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