逆转录病毒介导双靶区反义RNA在转基因小鼠肝细胞中的表达及对HBV DNA复制的影响
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张 政, 席宏丽, 公维波, 李文刚, 于 敏,
乙型肝炎病毒;反义RNA;转基因小鼠;HBVDNA,逆转录病毒介导双靶区反义RNA在转基因小鼠肝细胞中的表达及对HBVDNA复制的影响,张政,席宏丽,公维波,李文刚,于敏,曾争,徐小元,通讯作者:
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张政, 席宏丽, 公维波, 李文刚, 于敏, 曾争, 徐小元, 北京大学第一医院感染科 北京市 100034
国家自然科学基金资助项目, No. 30271182
通讯作者: 徐小元, 100034, 北京市大红罗厂街8号, 北京大学第一医院感染科. yangpin@public3.bta.net.cn
电话: 010-66551122-2311
收稿日期: 2006-06-10 接受日期: 2006-07-10
Retrovirus-mediated expression of dual-target antisense RNA in liver cells of transgenic mice and its influence on replication of hepatitis B virus
Zheng Zhang, Hong-Li Xi, Wei-Bo Gong, Wen-Gang Li, Min Yu, Zheng Zeng, Xiao-Yuan Xu
Zheng Zhang, Hong-Li Xi, Wei-Bo Gong, Wen-Gang LI, Min Yu, Zheng Zeng, Xiao-Yuan Xu,Department of Infectious Diseases, the First Hospital of Peking University, Beijing 100034, China
Supported byNational Natural Science Foundation of China, No. 30271182
Correspondence to:Xiao-Yuan Xu, Department of Infectious Diseases, the First Hospital of Peking University, Beijing 100034, China. yangpin@public3.bta.net.cn
Received:2006-06-10 Accepted:2006-07-10
Abstract
AIM: To detect the expression of X and P region dual-target antisense RNA of hepatitis B virus (HBV) mediated by ratroviral vector in the liver cells of transgenic mice and its effect on the replication of HBV DNA.
METHODS: The recombinant retroviral vector plasmids expressing dual-target antisense RNA complementary to HBV X and P region were constructed and then injected into the transgenic mice. The experimental mice were divided into blank, pLXSN, pLXSN-asX, pLXSN-asP, pLXSN-asXP, and pLXSN-seXP group. The total RNA, extracted from the mice at the 8th wk, was reversely transcribed, and then the cDNA was amplified with polymerase chain reaction (PCR). Thereafter, agarose gel electrophoresis, sequencing analysis and fluorescent PCR were performed. The replication of HBV DNA was tested by fluorescence quantitative PCR.
RESULTS: The HBV dual-target antisense RNA was expressed in the liver cells of mice and with high efficiency ......
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