炎症性肠病肠上皮细胞基质金属蛋白激酶对NAP-2的影响
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吕小平, 詹灵凌, 姜海行, 唐国都
炎症性肠病;基质金属蛋白激酶;中性白细胞激肽-2;血小板基础蛋白,吕小平,姜海行,唐国都,詹灵凌,吕小平,通讯作者,Effectofmatrix
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吕小平, 姜海行, 唐国都, 广西医科大学第一附属医院消化内科 广西壮族自治区南宁市 530021
詹灵凌, 广西医科大学第一附属医院临床实验中心 广西壮族自治区南宁市 530021
吕小平, 副教授, 硕士研究生导师, 2003年英国伦敦大学作访问学者, 主要从事炎症性肠病发病机制研究.
广西省自然科学基金资助项目, No. 桂科基0143059; No. 桂科攻0592007-1E
通讯作者: 吕小平, 530021, 广西壮族自治区南宁市, 广西医科大学第一附属医院消化内科. lxxp58@hotmail.com
电话: 0771-5356501
收稿日期: 2006-06-13 接受日期: 2006-06-23
Effect of matrix metalloproteinase on the neutrophil chemokine neutrophil activating peptide-2 of intestinal epithelial cells in inflammatory bowel disease
Xiao-Ping Lv, Ling-Ling Zhan, Hai-Xing Jiang, Guo-Du Tang
Xiao-Ping Lv, Ling-Ling Zhan, Hai-Xing Jiang, Guo-Du Tang,Department of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
Supported bythe Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 0143059, 0592007-1E
Correspondence to:Xiao-Ping Lv, Department of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China. lxxp58@hotmail.com
Received:2006-06-13 Accepted:2006-06-23
Abstract
AIM: To investigate the effect of matrix metalloproteinases (MMPs) on the neutrophil chemokine neutrophil activating peptide-2 (NAP-2) of intestinal epithelial cells and its relationship with the pathogenesis of inflammatory bowel disease (IBD).
METHODS: Caco-2 human colonic carcinoma cells and human CCD-18 colonic myofibroblast cells were incubated for 24 h at 37℃ with 50 mL/L CO2 before being treated with human recombinant MMP-3, IL-1β, the MMP inhibitors doxycyclin, CT1399, CT1847 and monoclonal anti-human NAP-2 neutralizing antibody, respectively. The cultured cells were tested by chemotaxis assay. PBP mRNA and MMPs mRNA in Crohn’s disease and ulcerative colitis tissues and cultured cells were examined by reverse transcription-polymerase chain reaction (RT-PCR) ......
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