【摘要】 目的 从分子水平探测中药治疗肝纤维化的作用机制。 方法 利用CCL4复合因素建立SD大鼠肝纤维化模型。实验动物分成正常对照组、模型组、中药治疗组3组，分别检测各组生化、肝纤维化指标和肝组织中ALR mRNA表达变化。 结果 ALT、AST、A/G、HA、PCⅢ、LN、PLD各指标:模型组均高于对照组(P<0.01)，中药治疗组低于模型组(P<0.01)，且HA、PCⅢ、LN、PLD的降低与病理组织反应肝纤维化的好转程度一致;肝组织中ALR mRNA的相对表达量:中药治疗组、模型组明显高于对照组(P<0.01)，中药治疗组高于模型组(P<0.01)。 结论 本中药方剂可显著升高肝纤维化大鼠肝组织ALR mRNA表达。

    【关键词】 基因表达调控

    【Abstract】 Objective To study the molecular mechanism of treatment by Chinese herbs on the hepat-ic fibrosis of rats.Methods Hepatic fibrosis model of rats was established with CCL4.Then the rats were divided into control，model and Chinese herbs treated groups.The level of ALR mRNA expres-sion，biochemical and hepatic fibrosis parameters were observed from different groups.Results The concentrations of ALT，AST，A/G，HA，LN，PLD and PCⅢin model group were higher than those in control group(P<0.01);those in Chinese herbs treated group were lower than in model group(P<0.01).The decrease of the levels of HA，LN，PLD and PCⅢin Chinese herbs treated group were cor-related with the improvement of hepatic fibrosis observed by pathology.The level of ALR mRNA ex-pression both in Chinese herbs treated group and model group were significantly higher than that in con-trol group(P<0.01).After treatment by Chinese herbs ......