白癜风；聚合酶链反应；噬菌体抗体库；自身抗体_

应用细胞内PCR技术构建及筛选白癜风噬菌体抗体库

http://www.100md.com 《第四军医大学学报》 2006年第16期

白癜风；聚合酶链反应；噬菌体抗体库；自身抗体,,白癜风；聚合酶链反应；噬菌体抗体库；自身抗体,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Construction and screening of human vitiligo phage autoantibody library based on incell PCR

    LIU Ling, TIAN YanLi, LI ChunYing, GAO TianWen

    Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To obtain in situ pairing of the variable region genes of the heavy and light chains of vitiligo patients lymphocytes, then construct and screen a human ScFv phage antibody library. METHODS: We amplified in situ pairing of the variable region genes of the immunoglobulin heavy and light chains using the incell PCR together with Crerecombination, constructed and panned a human ScFv phage antibody library, and screened it with pigment cell suspension, then detected the activity of positive clones. RESULTS: We constructed an antibody library of 3×l06 in size, and obtained one specific autoantibody against melanocyte membrane antigens. CONCLUSION: Incell PCR together with human phage antibody library may be useful tools for the study of vitiligo antibody gene family and the VH/VL pairing that occurs during the autoimmune process. ......

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