【摘要】 目的 研究健康成年Sprague-Dawley(SD)大鼠的前列腺、脾脏、肝脏等组织器官中免疫细胞的组成。 方法 对SD大鼠的前列腺、脾脏、肝脏等组织器官运用机械性淋巴细胞分离法(Mechiso)分离其淋巴细胞，并以流式细胞仪进行淋巴细胞的成分分析。 结果 前列腺组织中NKT细胞水平高于肝脏、脾脏组织3～10倍(P<0.05)，NK细胞高于脾脏5倍(P<0.05)，而辅助性T细胞(CD +4 αβTCR + )的水平低于肝脏及脾脏组织2倍(P<0.05);而且其CD +4 .CD +8 比值相对较低(P<0.05)。 结论 健康成年SD大鼠的前列腺组织中含有相对较多的NK细胞和NKT细胞，对抗御外来炎性侵害可能存在着重要作用。

    【关键词】 SD大鼠;NKT细胞;NK细胞

    The experimental study on the lymphocytes subsets in prostate of adult SD rats

    WANGWei，LIWenping，GUO Yuexian.

    Department of Urological Surgery，The Third Hospital of Hebei Medical University，Shijiazhuang050051

    【Abstract】 Objective To study the profile of lymphocyte subsets present in Sprague-Dawley(SD)rat's prostate，spleen and liver.Methods Lymphocyte isolation was done in8healthy，8to10week-old，Sprague-Dawley(SD)rat with the mechnical methods.Lymphocyte subsets were analyzed by flow cytometry and the results were com-pared.Results Levels of prostatic NKTwere increased3to10-fold compared with those found in other tissues(P<0.05) ......