Biological characteristics of placentaª²derived adherent cells

    SUN Nianª²Zheng, SHEN Baiª²Jun

    Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012, China

    ¡¾Abstract¡¿ AIM: To isolate and culture human placentaª²derived adherent cells (hPDAC) in vitro and study their biological characteristics involving morphology, phenotype and differentiation. METHODS: Mononuclear cells were harvested after placenta tissue was digested by collagenase IV, seeded and cultured in lowª²glucose DMEM containing 100 mL/L FBS. Their surface markers were detected by flow cytometry; the growth curve was made; cells of 3 passages were induced to differentiate into osteoblasts with vitamin C, dexamethasone and sodium ¦Âª²glycerophosphate, and the induced cells were observed in the change of matrix mineralization with alizarin red staining; cells of 3 passages were induced to differentiate into lipocytes with dexamethasone and insulin, and the induced cells were observed with oil red O staining. RESULTS: By day 7-14, a few of adherent cells were observed and expanded the same as fibroblasts, and adherent cells were positive for CD29, CD44 and CD105, but negative for CD34, CD45, CD19. After 10ª²day induction, alizarin red staining showed the calcium mineralization and after 7 d, oil red O staining revealed the appearance of fat drops. CONCLUSION: The hPDAC was confirmed to have the osteogenic and adipogenic potentials, indicating that they could be regarded as an alternative source of stem cells. ......