Analysis of different protein expressions in different liver cell strains in vitro using SELDI

    DING Shouª²Yi, QIAN Dongª²Meng, MU Wenª²Feng, YAN Zhiª²Yong, SONG Xuª²Xia, WANG Bin

    Department of Microbiology, Medical College, Qingdao University, Qingdao 266021, China

    ¡¾Abstract¡¿ AIM: To examine the differential expressions of proteins in hepatoma cell line (HepG2),hepatoma cell line transfected by HBV (HepG2.2.15)compared with normal liver cell line(LO2) by using surfaceª²enhanced laser desorption/ionization timeª²ofª²flight mass spectrometry (SELDIª²TOFª²MS), so as to establish a foundation for further studying on the mechanism of hepatoma at the protein level. METHODS: The 3 types of cells above mentioned were cultured as general and collected when they were in good conditions. After cell disruption, SELDIª²TOFª²MS was employed to detect the differential expressions of proteomes of HepG2, HepG 2.2.15 and LO2. RESULTS: Ninetyª²one proteins were captured by WCX2 array. Compared with normal liver cell lines, in the segments from Mr 5000 to 15 000, proteins in the HepG2 and HepG2.2.15 showed apparent changes, in which 2 proteins¬ð expressions were increased in carcinoma cells, the other 5 decreased. Nine proteins in HepG2 were increased and 10 proteins in HepG 2.2.15 were increased. CONCLUSION: The SELDI ProteinChip technology can be a desired method in detecting the biological markers in the earlier period of hepatoma. This method is of convenience, high sensitivity, and good reproducibility. The biological tags of tissueª²specific proteins we found have a potential applying value in the early diagnosis of hepatoma. These tags are also meaningful in screening and identifying signal proteins from serum and tissue specimen in different types of hepatoma. Certain foundation has been established in studying the etiopathogenesis of hepatoma at the level of proteins, as well as the searching of new therapeutic target sites. ......