氧应激对大鼠肝星状细胞增殖的影响及还原型谷胱甘肽的抗氧化作用
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刘 梅, 陆伦根, 陈尉华, 窦爱霞, 房静远,
氧应激;肝星状细胞;增殖;还原型谷胱甘肽;大鼠刘梅,陆伦根,陈尉华,窦爱霞,房静远,曾民德,郑瑞丹.氧应激对大鼠肝星状细胞增殖的影响及还原型谷胱甘肽的抗氧化作用. 世界华人消化杂志2006
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刘梅, 陆伦根, 陈尉华, 窦爱霞, 房静远, 曾民德, 上海交通大学医学院附属仁济医院消化内科 上海市消化疾病研究所 上海市 200001
郑瑞丹, 解放军第175医院肝病治疗中心 福建省漳州市 363000上海市重点学科建设项目, No. Y0205
上海市科学技术委员会基金, No. 054009618
通讯作者: 陆伦根, 200001, 上海市, 上海交通大学医学院附属仁济医院消化内科, 上海市消化疾病研究所. lungenlu1965@yahoo.com
电话: 021-63364118 传真: 021-63112874
收稿日期: 2006-07-14 接受日期: 2006-07-31
Effects of oxidative stress on proliferation of rat hepatic stellate cells and antioxidation of reduced glutathione
Mei Liu, Lun-Gen Lu, Wei-Hua Chen, Ai-Xia Dou, Jing-Yuan Fang, Min-De Zeng, Rui-Dan Zheng
Mei Liu, Lun-Gen Lu, Wei-Hua Chen, Ai-Xia Dou, Jing-Yuan Fang, Min-De Zeng, Department of Gastroenterology, Renji Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Institute for Digestive Diseases, Shanghai 200001, China
Rui-Dan Zheng, Treatment Center for Liver Disease, Chinese PLA 175 Hospital, Zhangzhou 363000, Fujian Province, China
Suppport by Shanghai Leading Academic Discipline Project, No. Y0205, and the Grant from Shanghai Science and Technology Committee, No. 054009618
Correspondence to: Lun-Gen Lu, Departmentment of Gastroenterology, Renji Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Institute for Digestive Diseases, Shanghai 200001, China. lungenlu1965@yahoo.com
Received: 2006-07-14 Accepted: 2006-07-31
AbstractAIM:To explore the effects of oxidative stress on the proliferation of rat hepatic stellate cells and the antioxidation of reduced glutathione.
METHODS:Rat hepatic stellate cells were incubated with different concentrations of ferric nitrilotriacetic acid (Fe-NTa). With 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphennylterazolium bromide (MTT) colorimetric assay, the effects of Fe-NTa on the proliferation of hepatic stellate cells at 6, 12, 24 and 48 h was detected, and malondialdehyde (MDA) contents and superoxide dismutase (SOD) activity were also detected ......
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