SUN Hui1, LI Xiaoª²Miao1, SONG Baiª²Li1, LIU Xinª²Ping2, YAO Liª²Bo2, JI Qiuª²He1

    1Department of Endocrinology, Xijing Hospital, 2Department of Biochemistry and Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To observe the effect of metabolic correlation factors on ionic transport and cell apoptosis of proximal renal tubular epithelial cells at the cellular level through observing the effects of uric acid and insulin of different concentrations on cAMP and Na+ª²K+ª²ATPase in proximal renal tubular epithelial cell line LLCª²PK1. METHODS: Proximal renal tubular epithelial cells (LLCª²PK1) from pig were cultured for 24 h in the medium supplemented with uric acid at 0, 0.1, 0.2, 0.4 mmol/L and insulin at 0, 10-9, 10-8, 10-7 mol/L; and then were observed in the level of cAMP into LLCª²PK1 cells, the activity of Na+ª²K+ª²ATPase and the results of ionic transport. RESULTS: After LLCª²PK1 cells were exposed to different concentrations of uric acid and insulin for 24 h£¬in uric acid group the activity of cAMP,[Ca2+],[Na+]were significantly increased in a concentrationª²dependent manner, but Na+ª²K+ª²ATPase activity, [K+] dropped in highª²concentration insulin groups, [Ca2+] and [Na+] were significantly elevated,but cAMP, Na+ª²K+ª²ATPase and [K+]were decreased. CONCLUSION: Uric acid and insulin could cause the increase of the concentration of calcium ion in cell, which might lead to cell dropsy and even induce apoptosis. In addition, high concentration of uric acid and insulin could do damages to LLCª²PK1 coordinately. ......