冯霞 张光毅

    冯霞(附属医院中心实验室， 徐州 221002)；张光毅(徐州医学院 生物化学与分子生物学研究中心)

    关键词: 大鼠海马脑片；缺氧；氯胺酮；苄丙咯；Ca²⁺/CaM PKⅡ

    摘要：用离体孵育的大鼠海马脑片模型，研究了两类钙通道拮抗剂对缺氧海马脑片Ca²⁺/CaM PKⅡ活性的影响。结果如下：(1)缺氧30min导致大鼠海马脑片Ca²⁺/CaM PKⅡ活性降至对照组的16.4%；(2)100μmol/L氯胺酮可部分拮抗缺氧导致的酶活性下降，使之恢复至对照组的40.6%；(3)10μmol/L苄丙咯可使缺氧导致的酶活性下降恢复至对照组的35.8%；(4)15μmol/L苄丙咯和50μmol/L氯胺酮联合用药可产生协同作用，使缺氧导致的酶活性下降恢复至对照组的63.8%。由此得出结论：缺氧对海马脑片Ca²⁺/CaM PKⅡ活性的抑制作用与NMDA受体门控的钙通道和Na⁺/Ca²⁺交换通道异常开放有关。

    中图分类号：R349.92 文献标识码：A

    文章编号：1001-6325(2000)06-0085-04

    Effect of two types of Ca²⁺ channels antagonist on Ca²⁺/CaM PKⅡ

    in anoxic rat hippocampal slices

    FENG Xia ZHANG Guang-yi

    (The Research Centre of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002,China)

    Abstract: Effect of two types of Ca²⁺ channels antagonist on anoxia-induced inhibition of Ca²⁺/CaM PKⅡ activity were studied in vitro in a model of rat hippocampal slices. The results were as follows: 1)Ca²⁺/CaM PKⅡ activity values of rat hippocampal slices decreased to 16.4% of control at 30min of anoxia. 2)Inhibition of Ca²⁺/CaM PKⅡ activity of the slices induced by 30min of anoxia was partly antagonized by NMDA receptor antagonist Ketamine and Na⁺/Ca²⁺ exchanger antagonist Bepridil, and Ca²⁺/CaM PKⅡ activity values changed from 16.4% of control to 40.6% of control (100μmol/L Ketamine) and to 35.8% of control(10μmol/L Bepridil). 3)Combination of 50μmol/L Ketamine with 15μmol/L Bepridil synergetically antagonized Inhibition of Ca²⁺/CaM PKⅡ activity, and Ca²⁺/CaM PKⅡ activity values changed from 16.4% of control to 63.8% of control. According to our results, we suggested that NMDA receptor and Na⁺Ca²⁺ exchanger be involved in anoxia-induced Inhibition of Ca²⁺/CaM PKⅡ activity in rat hippocampal slices. ......