蛙皮素和生长抑素对人胃癌BGC-823细胞生长的影响及受体后信息传递
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徐秀英, 赵金满, 姜若兰, 宗志宏
蛙皮素;生长抑素;胃癌;环磷酸腺苷;蛋白激酶C,徐秀英,赵金满,姜若兰,宗志宏,徐秀英,通讯作者:,Effectsof
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徐秀英, 赵金满, 姜若兰, 中国医科大学第一临床医院消化科 辽宁省沈阳市 110001
宗志宏, 中国医科大学基础医学院生化教研室 辽宁省沈阳市 110001
徐秀英, 2000年北京医科大学医学博士, 副教授, 副主任医师, 主要从事消化疾病、尤其是胃肠道激素和胃癌的临床研究.
通讯作者: 徐秀英, 110001, 辽宁省沈阳市, 中国医科大学第一临床医院消化科. xxying0828@sina.com
收稿日期: 2006-07-20 接受日期: 2006-08-10
Effects of bombesin and somatostatin on proliferation of human gastric cancer cell line BGC-823 and their postreceptor signal transduction
Xiu-Ying Xu, Jin-Man Zhao, Ruo-Lan Jiang, Zhi-Hong Zong
Xiu-Ying Xu, Jin-Man Zhao, Ruo-Lan Jiang,Department of Gastroenterology, the First Clinical College of China Medical University, Shenyang 110001, Liaoning Province, China
Zhi-Hong Zong,Department of Biochemistry, Basic Medical College of China Medical University, Shengyang 110001, Liaoning Province, China
Correspondence to:Xiu-Ying Xu, Department of Gastroenterology, the First Clinical College of China Medical University, Shenyang 110001, Liaoning Province, China. xxying0828@sina.com
Received:2006-07-20 Accepted:2006-08-10
Abstract
AIM: To observe the effects of bombesin and somatostatin on the proliferation of human lowly-differentiated gastric cancer cell line BGC-823, and to determine the intracellular concentration of cyclic adenosine monophos-phate (cAMP), the activity of proteinkinase C (PKC) and the expression of PKC isoforms α,b1,b2 ande.
METHODS: BGC-823 cells were cultured in RPMI 1640 medium containing 100 mL/L FCS in the atmosphere of 50 mL/L carbon dioxide at 37℃, and then bombesin or/and somatostatin with different concentrations was added. The viability and proliferation were determined by Mosmann's method (MTT assay); the intracellular cAMP concentrations were detected by radioimmunoassay (RIA); the activity of PKC was determined by incorporation of [γ-32p]-ATP with exogenous substrate; and the expression of PKC isoforms was analyzed by Western blot ......
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