三氧化二砷逆转肝癌细胞株HepG2/ADM多药耐药的作用
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李 贺, 熊茂明, 孟翔凌, 耿小平
肝癌;多药耐药;三氧化二砷,李贺,熊茂明,孟翔凌,耿小平,李贺,通讯作者:,Reversingeffectofarsenictriox
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李贺, 熊茂明, 孟翔凌, 耿小平, 安徽医科大学第一附属医院普外科 安徽省合肥市 230022
李贺, 住院医师, 在读研究生, 主要从事肝胆疾病研究.
通讯作者: 熊茂明, 230022, 安徽省合肥市, 安徽医科大学第一附属医院普外科三西病区. xiongmd@yahoo.com.cn
电话: 0551-2322035 传真: 0551-3633742
收稿日期: 2006-07-17 接受日期: 2006-08-10
Reversing effect of arsenic trioxide on multidrug resistance of human hepatocellular carcinoma HepG2/ADM cells
He Li, Mao-Ming Xiong, Xiang-Ling Meng, Xiao-Ping Geng
He Li, Mao-Ming Xiong, Xiang-Ling Meng, Xiao-Ping Geng, Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China
Correspondence to:Mao-Ming Xiong, Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China. xiongmd@yahoo.com.cn
Received:2006-07-17 Accepted:2006-08-10
Abstract
AIM: To explore the reversing effect of arsenic trioxide (As2O3) on the multidrug resistance (MDR) of human hepatocellular carcinoma HepG2/ADM cells in vitro and its potential mechanism.
METHODS: MTT assay was used to test the toxicity of As2O3 and the chemosensitivity to chemotherapeutics in As2O3-treated HepG2 and HepG2/ADM cells. Flow cytometry was used to determine the concentration of intracellular adriamycin (ADM). The expression of MDR1 was measured by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: The inhibitory rates of HepG2 and HepG2/ADM cells caused by As2O3 were lower than 15% under the dose of 0.25 mg/L, and the IC50 were 1.02 and 1.34 mg/L, respectively. As2O3 at 0.2 mg/L partly overcame the MDR of HepG2/ADM cells. The reverse efficiencies (multiples) to ADM, cisplatin (CDDP), mytomycin (MMC), 5-fluororacil (5-FU) were 2.92, 3.09, 2.13 and 2.60, respectively. Furthermore, after exposure to 0 ......
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