Ss-A/Ro核蛋白60 ku亚单位反义核酸逆转胃癌细胞多药耐药
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韩全利, 张希东, 丁 杰, 金晓维, 杨 玲,
胃癌;Ss-A,Ro60ku亚单位;多药耐药;反义核酸,Ss-A,Ro核蛋白60ku亚单位反义核酸逆转胃癌细胞多药耐药,韩全利,张希东,金晓维,杨玲,丁杰,王新,张学庸,樊代明,韩全利
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韩全利, 张希东, 金晓维, 杨玲, 空军总医院干部病房三区 北京市 100036
丁杰, 王新, 张学庸, 樊代明, 第四军医大学西京医院全军消化病研究所 陕西省西安市 710032
韩全利, 2003年第四军医大学博士毕业, 主要从事胃癌多药耐药的研究.国家自然科学基金资助项目, No. 30371623
通讯作者: 丁杰, 710032, 陕西省西安市长乐西路15号, 第四军医大学西京医院全军消化病研究所. hanquanli@21cn.com
电话: 029-84775230 传真: 029-82539041
收稿日期: 2006-07-04 接受日期: 2006-08-10
Reversal of multidrug resistance in gastric cancer cell line by Ss-A/Ro ribonucleoprotein 60-ku subunit antisense nucleic acid
Quan-Li Han, Xi-Dong Zhang, Jie Ding, Xiao-Wei Jin, Ling Yang, Xin Wang, Xue-Yong Zhang, Dai-Ming Fan
Quan-Li Han, Xi-Dong Zhang, Xiao-Wei Jin, Ling Yang,Department of Geratology, General Hospital of Chinese PLA Air Force, Beijing 100036, China
Jie Ding, Xin Wang, Xue-Yong Zhang, Dai-Ming Fan,Department of Gastroenterology, Xijing Hospital, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Supported byNational Natural Science Foundation of China, No. 30371623
Correspondence to:Dr. Jie Ding, Department of Gastroenterology, Xijing Hospital, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. hanquanli@21cn.com
Received:2006-07-04 Accepted:2006-08-10
Abstract
AIM: To investigate the possible function of Ro 60 antisense nucleic acid in multidrug resistant cell line of gastric cancer.
METHODS: Ro 60 antisense eukaryotic expression vector was constructed using DNA recombination technique, then transfected into SGC7901-VCR by LipofectamineTM2000. Drug sensitivity assay was performed using MTT assay, and IC50 values of gastric cancer cells to chemotherapy drugs were calculated. The intracellular accumulation of adriamycin in gastric cancer cells was measured using fluorescence-activated cell sorting.
RESULTS: The expression level of Ro 60 in SGC7901-VCR cells was decreased after transfection with antisense genes ......
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