阿司匹林和塞莱昔布对幽门螺杆菌的体外影响
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马慧霞, 王蔚虹, 胡伏莲, 李 江
幽门螺杆菌;非甾体类消炎药;阿司匹林;塞莱昔布马慧霞,王蔚虹,胡伏莲,李江.阿司匹林和塞莱昔布对幽门螺杆菌的体外影响. 世界华人消化杂志2006;14(28)2747-2752
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马慧霞, 王蔚虹, 胡伏莲, 李江, 北京大学第一医院消化内科 北京西城区西什库大街8号 北京市 100034
马慧霞, 北京大学第一医院消化内科博士生, 从事非甾体消炎药对幽门螺杆菌作用的研究. 国家自然科学基金资助项目, No. 30470777
通讯作者: 王蔚虹, 100034, 北京市西城区西什库大街8号, 北京大学第一医院消化内科. wh2581@sohu.com
电话: 010-66551122-2581 传真: 010-66518105
收稿日期: 2006-07-26 接受日期: 2006-08-23
Effect of aspirin and celecoxib on Helicobacter pylori in vitro
Hui-Xia Ma, Wei-Hong Wang, Fu-Lian Hu, Jiang Li
Hui-Xia Ma, Wei-Hong Wang, Fu-Lian Hu, Jiang Li, Department of Gastroenterology, the First Hospital, Peking University, Beijing 100034, China
Supported by the National Natural Science Foundation of China, No. 30470777
Correspondence to: Dr. Wei-Hong Wang, Department of Gastroenterology, the First Hospital, Peking University, Beijing 100034, China. wh2581@sohu.com
Received: 2006-07-26 Accepted: 2006-08-23
AbstractAIM:To investigate the possible effects of aspirin and celecoxib on the growth, virulence factors and outer membrane protein of H pylori.
METHODS: Two standard strains of H pylori were treated with a series concentration of aspirin or celecoxib (a specific inhibitor of cyclooxygenase 2) and were incubated in brucella broth under microaerobic condition for 24 and 48 h. The growth and urease activity were assessed by viable colony counting and spectrophotometry. After 48-hour treatment, the supernatants were incubated with Hela cells to estimate vacuolating cytotoxin activity. The outer membrane protein ofH pylori was analyzed by SDS-PAGE.
RESULTS: Aspirin or celecoxib inhibited the growth of H pylori in a dose-dependent manner with the lowest inhibitory concentrations of 0.5 or 0.01 mmol/L, and the lethal concentrations of 2.0 and 0.04 mmol/L, respectively. Aspirin and celecoxib inhibited the urease activity and vacuolating cytotoxin activity of H pylori. The inhibitory effect of celecoxib on H pylori was superior to that of aspirin ......
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