蔬菜中苯甲酰脲类药物残留的测定方法研究
苯甲酰脲,残留分析,高效液相色谱,,苯甲酰脲，残留分析，高效液相色谱,1引言,2实验部分,3结果与讨论,References
摘 要 建立了一步有机溶剂提取、HPLC分离、紫外检测器检测7种苯甲酰脲类药物(除虫脲、灭幼脲、杀铃脲、氟铃脲、氟苯脲、氟虫脲和氟啶脲)在蔬菜中残留量的方法。考察了不同提取溶剂(乙酸乙酯和乙腈)的提取效率；研究了不同C18固相萃取小柱、活性碳、自制弗罗里硅土柱和GPC对蔬菜样品的净化效果；实验了不同的梯度淋洗程序分离7种药物。通过对黄瓜、大白菜、西红柿和包心菜4种蔬菜的4种添加水平和4次重复性实验，建立了一种以乙腈为提取溶剂和以弗罗里硅土柱为净化柱的高效液相色谱法测定7种苯甲酰脲类药物。该方法线性范围为：0.02～1.5mg/L，7种苯甲酰脲类药物的相关系数均大于0.999，其检出限为0.02～0.05 mg/kg(S/N=10)；在0.05～1.0 mg/kg之间的添加回收率为80 %～120%；相对标准偏差小于15%。方法完全符合残留分析的要求。关键词 苯甲酰脲，残留分析，高效液相色谱
Multiresidue Determination of Benzoylurea Insecticides
in Vegetable Matrices
He Hongmei, Wu Liqin, Zhang Hu, Zhu Jiahong, Xie Lei
(Institute of Quality and Standard on Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021)
Abstract Onestep extraction and high performance liquid chromatography (HPLC) with ultraviolet detection have been developed and validated for the determination of seven kinds of benzoylurea insecticides (diflubenzuron, chlorbenzuron, triflumuron, hexaflumuron, teflubenzuron, flufenoxuron and chlorfluazuron) residues in vegetable matrices including Chinese cabbage, tomato, cabbage and cucumber. The extraction efficiency of ethyl acetate and acetonitrile ......
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