蛋白微阵列免疫分析法用于海洋致病菌的定量检测
蛋白微阵列,荧光免疫法,海洋致病菌,,蛋白微阵列，荧光免疫法，海洋致病菌,1引言,2实验部分,3结果与讨论,References
摘 要 建立了一种用于水体中副溶血弧菌、河弧菌和大肠杆菌检测定量的蛋白微阵列免疫分析法。以Cy3标记免疫球蛋白(IgG)为探针，蛋白芯片为载体，对孵育反应的IgG浓度、反应时间和温度等条件进行了优化，结果表明：当IgG浓度为0.1 g/L在37℃下孵育60 min，可以获得理想的检测效果；在优化条件下，本方法中副溶血弧菌、河弧菌和大肠杆菌的检出限分别为9.9×104个/mL、9.3×104个/mL和 3.9×105个/mL；将本方法与ELISA进行了比较，结果表明，本方法具有快速、操作便捷、高通量检测等特点；并将本方法应用于海水中这3种致病菌的快速、准确地定量检测。关键词 蛋白微阵列，荧光免疫法，海洋致病菌
Quantitative Detection of Marine Pathogenic Bacteria Using Protein Microarray Immunoassay
Huang Rongfu，Zhuang Zhixia， Yan Qingpi，Li Jingxi， Chen Shi， Wang Xiaoru
1Department of Chemistry and The Key Laboratory of Analytical Sciences of the Ministry of Education，College of Chemistry and Chemical Engineering， Xiamen University， Xiamen 361005
2First Institute of Oceanography， Qingdao 266061
3Fishery college，Jimei University， Xiamen 361007
Abstract A protein microarray immunoassay method was established in the detection and quantification of E.coli, V. fluvialis and V. parahaemolyticus in water. Cy3rabbit immunoglobulin G (IgG) was used as a probe. 37℃, 60 min , 0.1 g/L of concentration of IgG. were testified to the optimized conditions in the incubation reaction. For E.coli, the limit of detection was 3.9×105cfu/mL, for V. fluvialis was 9.3×104 cfu/mL and for V. parahaemolyticus was 9.9×104 cfu/mL. Compared with enzyme linlced immunosorbent assay (ELISA), the high throughput, rapid and convenient detection of Marine Bacterial Pathogens was demonstrated in the optimized conditions and marine samples were analyzed subsequently using this method effectively. ......
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