离子液体_双水相_牛血清白蛋白_萃取分离_

离子液体双水相体系萃取分离牛血清白蛋白研究

http://www.100md.com 《分析化学》 2006年第10期

离子液体,双水相,牛血清白蛋白,萃取分离,,离子液体，双水相，牛血清白蛋白，萃取分离,1引言,2实验部分,3结果与讨论,References

     摘要建立了由亲水性离子液体四氟硼酸1甲基3丁基咪唑([Bmim]BF4 )和KH2PO4 形成的双水相体系萃取分离牛血清白蛋白(BSA)的新方法。研究了不同盐及盐的浓度、离子液体浓度以及蛋白质用量、溶液酸度、其它共存物质对双水相成相及BSA萃取率的影响，结果表明, 磷酸二氢钾盐浓度为80 g/L，离子液体浓度在160~240 mL/L，BSA的浓度为30~50 mg/L，溶液酸度在pH 4~8范围，离子液体双水相体系对BSA有较高的萃取率。用加入不同类型表面活性剂探讨了离子液体与蛋白质之间的作用。

    关键词离子液体，双水相，牛血清白蛋白，萃取分离

    Study on the Extraction Separation of BSA in

    Ionic Liquid Aqueous Twophase System

    Deng Fanzheng， Guo Dongfang

    (Department of Chemistry, HuaiBei Coal Industry Teacher′s College, Huaibei 235000)

    Abstract A new method to extract and separate Bovine Serum Albumin(BSA) in a new aqueous twophase system(ATPS) formed by a room temperature watersoluble ionic liquid [Bmim]BF4 (1butyl3methylimidazoliumtetrafluoroborate) and phaseforming salt KH2PO4 was studied. The effects of concentration of ionic liquid, salt and BSA, solution pH value and other coexistence sulstance on the extraction efficiency and the formation twophase were investigated systematically. With the solution pH value 4-8, the addition of BSA 30-50 mg/L, [Bmim]BF4 160-240 mL/L and KH2PO4 80 g/L, Ionic liquidATPS had the highest extraction efficiency.With different surfactant, the interaction of protein and ionic liquid were discussed initiatively. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/10/18/25/46/64.htm

您现在查看是摘要页，全文长 7700 字符。