土壤和植物中乙二胺二琥珀酸的反相液相色谱法测定
乙二胺二琥珀酸,衍生化,液相色谱法,,乙二胺二琥珀酸,衍生化，液相色谱法,1引言,2实验部分,3结果与讨论,References
摘 要 乙二胺二琥珀酸(EDDS)可应用于重金属污染土壤的强化植物修复或是土壤淋洗修复。但是现有的检测方法或过于复杂,或检出限太高。本研究建立了应用超声波萃取、硫酸铜衍生化和液相色谱，紫外检测器测定土壤、植物中EDDS含量的方法。其回收率为93%~118%，灵敏度较高；检出限达1.5 μmol/L；重现性好，不受其它多种共存离子的干扰。关键词 乙二胺二琥珀酸,衍生化，液相色谱法
Determination of Ethylenediaminedisuccinic Acid in Soil and Plant Using Reversed Phase High Performance Liquid Chromatography
Sun Xiaofeng, Wu Longhua, Luo Yongming
1Soil and Environment Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008
2Graduate School of the Chinese Academy of Sciences, Beijing 100049
Abstract Ethylenediaminedisuccinic acid (EDDS) can be used in chelate enhanced phytoremediation or leaching remediation for heavy metalpolluted soil. But current method have a very high detection limit or complex process. This study describes the development of a method for determining EDDS in soil and plant at low concentration s by high performance liquid. After short sample pretreatment, the samples extracted by supersonic and derived with copper sulfate were analyzed by reversed phase liquid chromatography. The mobile phase is 0.03 mol/L acetate acetic acid buffer of pH 4.0 with counter ion. Ultraviolet absorbance detection was applied at 254 nm. The method is simple and a low limit of detection (1.5 μmol/L). The correlation coefficient exceeded 0.99 and the recovery efficiency is between 93%-118%. It is reliable and the interference from other metal ions was eliminated. ......
您现在查看是摘要页，全文长 10343 字符。