【摘要】 目的 从基因水平调查遵义汉族人群HLA-DQBl等位基因频率，并了解其多态性分布状况。方法 应用聚合酶链反应-序列特异性引物(PCR-SSP)方法对遵义汉族109名健康人群进行等位基因分型。结果 检出14个HLA-DQB1等位基因，遵义汉族人HLA-DQB1等位基因频率分布为HLA-DQB1 *0303> *0301> *0601> *0502> *050301=*0401> *0201 > *0302> *050101=*0202> *0602> *0402=060401> *0609。结论 得到了遵义汉族人HLA-DQB1等位基因频率分布的可靠资料，为我国疾病相关性研究、人类学研究提供了可靠的遗传学数据。

    【关键词】 遵义；HLA-DQBl；基因频率；聚合酶链反应-序列特异性引物

    Research of HLA-DQB1 gene polymorphism on the Hans of Zunyi

    FENG Ji-hong，SUN Wan-bang，LUO Jun-min，et al.Zhuhai Campus of Zunyi Medical College，Guangdong Zhuhai 519041，China

    【Abstract】 Objective To research HLA-DQB1 allele frequency and polymorphism in the Hans of Zunyi area at gene level.Methods Polymerase chain reaction-sequence specific primers (PCR-SSP) were used to type HLA-DQB1 gene polymorphism of 109 unrelated healthy Han individuals of Zunyi area.Results We inspected 14 alleles of HLA-DQB1and a series of gene frequency(GF)distribution was obtained as following： HLA-DQB1 *0303> *0301> *0601> *0502> *050301=*0401> *0201> *0302> *050101=*0202> *0602> *0402=060401> *0609.Conclusion This study has obtained a reliable data of HLA-DQB1 allele frequency distribution in the Hans of Zunyi area ......