Comparison between STO cells and hELF used as feeder layers of embryonic germ cells

    LIU Yongª²Gang, LI Fangª²Fei, CHEN Diª²Long, WANG Shaª²Li, WANG Yaª²Ping

    Department of Histology and Embryology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China

    ¡¾Abstract¡¿ AIM: To compare the influence on the growth of human embryonic germ (EG) cells by SIM mouse embryoª²derived thioguanine and ouabain resistant (STO) cells and human embryonic lung fibroblasts(hELF)respectively as feeder layers and the changes of the two kinds of the feeder layers themselves processed with mitomycin C. METHODS: We isolated and cultured hELF of 3-4 month embryos. The changes of the two kinds of the feeder layers themselves treated with mitomycin C were observed. STO and hELFs erved as feeder layer to cultivate human EG cells.The formation rate of embryonic germ cell colony and the expression of EG cell surface markers(SSEAª²1, SSEAª²4) expression were detected. to discuss the influence on human EG cell growth. RESULTS: HELF treated by mitomycin C survived longer than STO cells treated by mitomycin C did. There was no obvious difference between the two kinds of feeder layers in the formation rate and immunologic markers (SSEAª²1, SSEAª²4) of EG cell colony. CONCLUSION: HELF as feeder layers is better than STO cells in culturing EG cells. ......