survivin_mRNA;_端粒酶活性；喉癌;

喉癌中survivin mRNA与端粒酶活性的关系

http://www.100md.com 《现代医学》 2006年第5期

survivin,mRNA;,端粒酶活性；喉癌;,荧光定量PCR;,酶联免疫端粒重复序列扩增法,,]survivin,mRNA;,端粒酶活性；喉癌;,荧光定量PCR;,酶联免疫端粒重复序列扩增法

     [摘要]目的通过测定23例喉癌患者喉癌组织的端粒酶活性和凋亡抑制因子survivin mRNA表达水平，探讨端粒酶活性和survivin mRNA表达水平这两个指标之间的关系。方法采用Taqman技术建立荧光实时定量FQPCR方法检测喉鳞癌组织的survivin mRNA；应用TRAPELISA法测定喉癌组织的端粒酶活性。结果喉癌组织中survivin mRNA的表达水平与端粒酶活性具有相关性，呈正相关(r=0591， P=0003)。结论 survivin可能是使端粒酶活性增高的细胞因子。

    [关键词]survivin mRNA; 端粒酶活性；喉癌; 荧光定量PCR; 酶联免疫端粒重复序列扩增法

    Relationship of survivin mRNA and telomerase activity

    in the larynx carcinoma

    FENG Xu1，HUANG Zhichun 1，HE Xiaosong 2,ZHANG Fengbing1，SUN Baobin1，JI Hui1(1Department of Otolarynology,Zhongda Hospital, Southeast University，Nanjing 210009, China;

    2 Biochemistry Laboratory, Jiangsu Cancer Institute, Nanjing 210009,China)

    Abstract: Objective To measure the expression of survivin mRNA and telomerase activity in larynx carcinoma, and analyse the relationship between survivin mRNA and telomerase activityMethods Expression of survivin mRNA and telomerase activity were analysed in 23 patients with larynx carcinoma by FQPCR and TRAPELISAResult Expression of survivin mRNA in larynx carcinoma had positive correlation to telomerase activity(r=0591, P=0003)Conclusion survivin may be one of the cell factors to increase telomerase activity ......

百拇医药网 http://www.100md.com/html/DirDu/2006/11/03/27/30/30.htm

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