乙型肝炎病毒;_脱氧核糖核酸;_荧光定量聚合酶链反应;

HBV DNA与HBVm对比分析

http://www.100md.com 《河北北方学院学报》 2006年第1期

乙型肝炎病毒;,脱氧核糖核酸;,荧光定量聚合酶链反应;,血清标志物;,酶联免疫吸附试验,,乙型肝炎病毒;,脱氧核糖核酸;,荧光定量聚合酶链反应;,血清标志物;,酶联免疫吸附试验,HBVDNA与H

     【摘要】目的：探讨荧光定量PCR检测HBV DNA与HBVm的关系。方法：采用荧光定量PCR法和酶联免疫吸附试验(ELISA)对159份HBVm 8种不同阳性模式及67份HBVm全阴模式血清进行HBV DNA检测。结果： HBsAg、HBcAb、HBeAe阳性者HBV DNA阳性率为8983%； HBsAg、HBcAb、HBeAb阳性者HBV DNA阳性率为2881%； HBsAb、HBcAb、HBeAb阳性者HBV DNA阳性率为1666%； HBVm全阴性者HBV DNA阳性率为1791%。结论：荧光定量PCR法检测HBV DNA是乙型肝炎病毒复制最直接可信的指标，可反映HBV真实感染和复制状态，特别是低复制状态，明显优于ELISA法检测HBVm，具有重要临床意义。

    【关键词】乙型肝炎病毒; 脱氧核糖核酸; 荧光定量聚合酶链反应; 血清标志物; 酶联免疫吸附试验

    Comparative Analysis between HBV DNA by FQPCR and HBVm by ELISA

    LIU Jinlu, LI Caiqing, LU Cheng, et al

    The First Affiliated Hospital， Hebei North University, Zhangjiakou, 075000, Hebei China

    【ABSTRACT】Objective: To investigate the relationship between HBVDNA determined by the polymerase chain reaction (PCR) assay and HepatitisB markers by ELISA method.Methods: The serum samples from 159 cases with 8 different positive models and 67 cases with all negative models were tested by Fluorescence quantitative PCR assay (FQPCR) and also by ELISA to compare the results.Results: Among the patients,the positive rate of HBVDNA for the patients with positive HBsAg、HBcAB and HBeAe was 8983%;the positive rate of HBVDNA for the patients with positive HBsAg、HBcAb and HBsAb was 2881%;the positive rate of HBVDNA for the patients with positive HBsAb、HBcAb and HBeAb was 1666%;the positive rate of HBV DNA for the patients with all negative HBVm was 1791%.Conclusione: HBVDNA determined by PCR assay is much more corrct and sensitive than HBVmarkers by ELISA assay.FQPCR could be used as a excellent monitor for the state of HBV irfection and its complication, especially for the state of low complication. ......

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