双氧水对鼻咽癌细胞生长特性的影响及其机制的初步研究
人低分化鼻咽癌细胞；H2O2；肿瘤相关基因；细胞培养；肿瘤治疗,,人低分化鼻咽癌细胞；H2O2；肿瘤相关基因；细胞培养；肿瘤治疗,1材料与方法
【摘要】目的: 观察H2O2对人低分化鼻咽癌细胞(CNE2Z)生长、增殖能力的影响，并探讨其可能机制。方法:用MTT及平板克隆形成实验、Western Blot等检测H2O2诱导CNE2Z后增殖能力、EGR1、p53、p16、Cyclin D1的表达。结果：不同浓度H2O2可不同程度地抑制CNE2Z细胞的生长增殖能力，同一时间点各实验组细胞抑制率相比差异有统计学意义，抑制率与H2O2浓度间表现出明显的剂量效应关系。平板克隆形成实验的抑制率较MTT更明显。CNE2Z无EGR1、p16蛋白表达，可见p53、Cyclin D1蛋白表达；诱导培养后EGR1、p53、 Cyclin D1蛋白表达明显增加。结论：一定浓度的H2O2能抑制CNE2Z细胞生长，其作用可能与EGR1及p53蛋白表达增强有关；Egr1基因在CNE2Z中具有可诱导性，在肿瘤治疗中有一定的应用前景。【关键词】 人低分化鼻咽癌细胞；H2O2；肿瘤相关基因；细胞培养；肿瘤治疗
The effects and possible mechanisms of H2O2 on the proliferation
of nasopharyngeal carcinoma cell line (CNE2Z)
YAO Yunhong, ZHONG Shoujun, XIONG Hui
(1. Department of Pathology, Guangdong Medical College, Zhanjiang 524023， China；
2. Department of Pathology, Zhongshan Women and Children Hospital, Zhongshan 528403， China)
【Abstract】 Objective: To observe the effects of H2O2 on the proliferation of nasopharyngeal carcinoma cell line (CNE2Z) and study the possible mechanisms. Methods: The CNE2Z cells were treated with H2O2; the assays of MTT and plate colony formation were used to measure the ability of proliferation of CNE2Z cells; the western blot was used to detect the protein levels of Egr1, p53, p16 and cyclin D1. Results: In a dosage dependent manner, H2O2 inhibited the proliferation of CNE2Z cells, shown by both the MTT and plate colony formation assays with stronger displaying in the plate colony formation assay; without treatment of H2O2 CNE2Z cells express slight p53 and cyclin D1 proteins but no detectable Egr1 and p16 proteins; after treatment with H2O2, CNE2Z express significant higher proteins of Egr1, p53 and cyclin D1 with no detectable change p16 protein. Conclusion: H2O2 might inhibit the proliferation of CNE2Z through increasing the expressions of Egr1 and p53 proteins; since Egr1 expression was inducible, Egr1 might be a target in the tumor therapy. ......
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