KDR启动子驱动双自杀基因靶向杀伤人胃癌细胞的作用
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李 强, 黄宗海, 俞金龙, 苏国强, 厉 周,
人胃癌细胞;KDR启动子;自杀基因治疗;腺病毒;旁观者效应,李强,黄宗海,俞金龙,苏国强,厉周,周光军,李强,通讯作者:,Adenoviru
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李强, 黄宗海, 俞金龙, 苏国强, 厉周, 周光军, 南方医科大学珠江医院普外科 广东省广州市 510282
李强, 南方医科大学博士生, 副教授, 副主任医师, 主要从事胃肠道肿瘤的临床与基础研究.
国家863计划资助项目, No. 2001AA217171
广东省自然科学基金项目, No. 013072
通讯作者: 黄宗海, 510282, 广东省广州市, 南方医科大学珠江医院普外科. drhuangzh@yahoo.com.cn
电话: 020-61643213 传真: 020-61643213
收稿日期: 2006-07-21 接受日期: 2006-09-29
Adenovirus-mediated double suicide gene driven by KDR promoter selectively kills human gastric adneocarcinoma cell line SGC7901
Qiang Li, Zong-Hai Huang, Jin-Long Yu, Guo-Qiang Su, Zhou Li, Guang-Jun Zhou
Qiang Li, Zong-Hai Huang, Jin-Long Yu, Guo-Qiang Su, Zhou Li, Guang-Jun Zhou,Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
Supported bythe Foundation of National “863” Program, No. 2001AA217171, and the Natural Science Foundation of Guangdong Province, No. 013072
Correspondence to:Zong-Hai Huang, Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China. drhuangzh@yahoo.com.cn
Received:2006-07-21 Accepted:2006-09-29
Abstract
AIM: To study effect of adenovirus (Ad)-mediated fusion gene system driven by KDR promoter on the proliferation of human gastric adenocarcinoma cell line SGC7901.
METHODS: SCG7901 cells (with KDR expression) and HepG2 cells (without KDR expression) were both transfected with AdEasy-KDR-CDglyTK vector, followed by treatment of the prodrugs 5-fluorocytosine (5-FC) and/or ganciclovir (GCV) at different concentrations. The killing and bystander effects on both kinds of cells were evaluated.
RESULTS: The green fluorescent protein (GFP) was observed in 95% of the infected SCG7901 and HepG2 cells when the multiple of infection (MOI) was 100. No significant difference was found in the characteristics of growth between the transfected and non-transfected SCG7901 cells (t = 0 ......
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