伯氏疟原虫；恶性疟原虫；顶端膜抗原1；转染_

恶性疟原虫顶端膜抗原1基因转染伯氏疟原虫模型的建立

http://www.100md.com 《第四军医大学学报》 2006年第19期

伯氏疟原虫；恶性疟原虫；顶端膜抗原1；转染,,伯氏疟原虫；恶性疟原虫；顶端膜抗原1；转染,0引言,1材料和方法,2结果,3讨论,【参考文献】

     CHEN Jun, MIAO Jun, LIU ZhongXiang, LI ShuMei, XUE CaiFang

    Department of Etiology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To establish the model of Plasmodium berghei (P. berghei) transfected with Plasmodium falciparum (P. falciparum) apical membrane antigen1 (AMA1) gene. METHODS: The transfection plasmid pDB.DTm.DB./AB.AF.DB. containing P.falciparum AMA1 was enzymed by BglI. Blood stages of P.berghei were cultured in vitro, and mature schizonts were purified. Parasites transfected by electroporation were injected into tail veins of mice. Transfected parasites were selected by treating mice with pyrimethamine. DNA of transfected, resistant parasites was isolated and analyzed by PCR.RESULTS: The gene encoding P.falciparum AMA1 was amplified by PCR in the transfected parasites. CONCLUSION: The model of P.berghei transfected with P.falciparum AMA1 gene was established, which provided a basis for further study. ......

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