金属硫蛋白对仔猪抗氧化功能及SOD基因表达的影响
金属硫蛋白;抗氧化酶;基因表达;仔猪,,金属硫蛋白;抗氧化酶;基因表达;仔猪,0引言,1材料和方法,2结果,3讨论,【参考文献】
LI LiLi1, LIU YunHua2, HOU DeXing3, YIN YuLong1, ZHANG Bin2, HOU ZhenPing1, QIU XiMin41Institute of Subtropical Agriculture, Chinese Academy of Science, Changsha 410125,China, 2College of Animal Science and Technology, Hunan Agricultural University,Changsha 410128,China, 3Department of Biochemical and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima 8908580, Japan, 4Medical College,Hunan Normal University,Changsha 410006,China
【Abstract】 AIM: To inverstigate the effects of exogenous Znmetallothionein (ZnMT) on antioxidative function and gene expression of SOD in weaning piglets. METHODS: Eighteen piglets (Duroc×Landrace×Yorkshire) were selected and divided into 3 groups (1, 2 and 3) randomly. The piglets were sport to produce stress. ZnMT of piglet liver dissolved in physiological saline were then injected into the piglets of group 1, 2 and 3 with the concentrations of 0, 0.8, 1.6 mg/kg, respectively. Three and 6 h later, 3 piglets were selected from each group randomly and slaughtered to get liver samples. The biochemical indexes related to antioxidation and the level of SOD gene expression in liver were determined. RESULTS: After ZnMT injection, the activities of SOD and GSHPX increased significantly (P<0.05) ......
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